A sense of closeness: protein detection by proximity ligation

被引:54
作者
Gullberg, M [1 ]
Fredriksson, S
Taussig, M
Jarvius, J
Gustafsdottir, S
Landegren, U
机构
[1] Rudbeck Lab, Beijer Lab, Dept Genet & Pathol, SE-75185 Uppsala, Sweden
[2] Babraham Inst, Technol Res Grp, Cambridge CB2 4AT, England
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1016/S0958-1669(02)00011-3
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Highly specific and sensitive procedures will be required to evaluate proteomes. Proximity ligation is a recently introduced mechanism for protein analysis. In this technique, the convergence of sets of protein-binding reagents on individual target molecules juxtaposes attached nucleic acid sequences. Through a ligation reaction a DNA reporter sequence is created, which can be amplified. The procedure thus encodes detected proteins as specific nucleic acid sequences in what may be viewed as a reverse translation reaction.
引用
收藏
页码:82 / 86
页数:5
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