Development and Evaluation of Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP) Assay Coupled with a Portable Device for Rapid Diagnosis of Ebola Virus Disease in Guinea

被引：75

作者：

Kurosaki, Yohei ^{[1
]}

Magassouba, N'Faly ^{[2
,3
,4
]}

Oloniniyi, Olamide K. ^{[1
,5
,6
]}

Cherif, Mahamoud S. ^{[1
]}

Sakabe, Saori ^{[1
,5
,6
]}

Takada, Ayato ^{[7
]}

Hirayama, Kenji ^{[1
,5
,6
]}

Yasuda, Jiro ^{[1
,5
,6
]}

机构：

[1] Nagasaki Univ, Inst Trop Med NEKKEN, Nagasaki 852, Japan

[2] Lab Fievres Hemorrag Guinee, Conakry, Guinea

[3] Serv Malad Infectieuses & Trop, Hop Natl Donka, Conakry, Guinea

[4] Univ Gamal Abdel Nasser, Conakry, Guinea

[5] Nagasaki Univ, Grad Sch Biomed Sci, Nagasaki 852, Japan

[6] Nagasaki Univ, Program Nurturing Global Leaders Trop & Emerging, Nagasaki 852, Japan

[7] Hokkaido Univ, Res Ctr Zoonosis Control, Sapporo, Hokkaido, Japan

来源：

PLOS NEGLECTED TROPICAL DISEASES | 2016年 / 10卷 / 02期

关键词：

TRANSMISSION; EVOLUTION;

D O I：

10.1371/journal.pntd.0004472

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Given the current absence of specific drugs or vaccines for Ebola virus disease (EVD), rapid, sensitive, and reliable diagnostic methods are required to stem the transmission chain of the disease. We have developed a rapid detection assay for Zaire ebolavirus based on reverse transcription-loop-mediated isothermal amplification (RT-LAMP) and coupled with a novel portable isothermal amplification and detection platform. The RT-LAMP assay is based on primer sets that target the untranscribed trailer region or nucleoprotein coding region of the viral RNA. The test could specifically detect viral RNAs of Central and West African Ebola virus strains within 15 minutes with no cross-reactivity to other hemorrhagic fever viruses and arboviruses, which cause febrile disease. The assay was evaluated using a total of 100 clinical specimens (serum, n = 44; oral swab, n = 56) collected from suspected EVD cases in Guinea. The specificity of this diagnostic test was 100% for both primer sets, while the sensitivity was 100% and 97.9% for the trailer and nucleoprotein primer sets, respectively, compared with a reference standard RT-PCR test. These observations suggest that our diagnostic assay is useful for identifying EVD cases, especially in the field or in settings with insufficient infrastructure.

引用

页数：12

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