Phenotypic and molecular characterization of a Brucella strain isolated from a minke whale (Balaenoptera acutorostrata)

被引:75
作者
Clavareau, C
Wellemans, V
Walravens, K
Tryland, M
Verger, JM
Grayon, M
Cloeckaert, A
Letesson, JJ
Godfroid, J
机构
[1] Ctr Etud & Rech Vet & Agrochim, B-1180 Brussels, Belgium
[2] Norwegian Coll Vet Med, Dept Arctic Vet Med, N-9005 Tromso, Norway
[3] INRA, Pathol Infect & Immunol Lab, F-37380 Nouzilly, France
[4] Fac Univ Notre Dame Paix, Unite Immunol Microbiol, B-5000 Namur, Belgium
来源
MICROBIOLOGY-SGM | 1998年 / 144卷
关键词
Brucella sp; brucellosis; minke whale (Balaenoptera acutorostrata);
D O I
10.1099/00221287-144-12-3267
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Isolation of Brucella spp. in marine mammals has been reported during the past several years. A Brucella strain from the spleen and liver of a minke whale (Balaenoptera acutorostrata) was isolated. Conventional typing methods indicated that this isolate was related to the genus Brucella but did not match the profiles of any known Brucella species or biovar. Successful PCR amplification of the Brucella rrs-rrl spacer sequence and of the insertion sequence IS6501 also indicated that the minke whale strain was related to the genus Brucella. In addition, the rrs gene of this strain shared a very high degree of nucleotide identity (>98%) with published Brucella spp. rrs sequences. However, RFLP studies using an IS6501-specific probe showed a unique profile for this strain in comparison with the profiles of the six known Brucella species. Moreover, analysis of the omp2 locus by Southern hybridization using omp2a- and omp2b-specific probes, and by DNA sequencing showed that minke whale isolate possesses two copies or me omp2b gene instead of one omp2a and one omp2b gene copy or two copies of the omp2a gene described in the six known Brucella species. Thus, molecular typing methods showed that this isolate is clearly distinct from all other known Brucella species and strains. The specific molecular features of this minke whale Brucella isolate raise questions about the lineage between the Brucella strains isolated from marine mammals and the Brucella species isolated from terrestrial mammals.
引用
收藏
页码:3267 / 3273
页数:7
相关论文
共 34 条
[1]  
ALTON G. G., 1988, TECHNIQUES BRUCELLOS
[2]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[3]  
Ausubel F., 1990, CURRENT PROTOCOLS MO
[4]   Nucleotide sequence and expression of the gene encoding the major 25-kilodalton outer membrane protein of Brucella ovis: Evidence for antigenic shift, compared with other Brucella species, due to a deletion in the gene [J].
Cloeckaert, A ;
Verger, JM ;
Grayon, M ;
Zygmunt, MS ;
Grepinet, O .
INFECTION AND IMMUNITY, 1996, 64 (06) :2047-2055
[5]  
Cloeckaert A, 1996, FEMS MICROBIOL LETT, V145, P1, DOI 10.1016/0378-1097(96)00373-4
[6]   RESTRICTION SITE POLYMORPHISM OF THE GENES ENCODING THE MAJOR 25 KDA AND 36 KDA OUTER-MEMBRANE PROTEINS OF BRUCELLA [J].
CLOECKAERT, A ;
VERGER, JM ;
GRAYON, M ;
GREPINET, O .
MICROBIOLOGY-UK, 1995, 141 :2111-2121
[7]  
Corbel M.J., 1984, Bergey Manual of Systematic Bacteriology, P377
[8]   SEROLOGIC SURVEY FOR BRUCELLOSIS IN FERAL SWINE, WILD RUMINANTS, AND BLACK BEAR OF CALIFORNIA, 1977 TO 1989 [J].
DREW, ML ;
JESSUP, DA ;
BURR, AA ;
FRANTI, CE .
JOURNAL OF WILDLIFE DISEASES, 1992, 28 (03) :355-363
[9]   CHARACTERISTICS OF A BRUCELLA SPECIES FROM A BOTTLE-NOSED-DOLPHIN (TURSIOPS-TRUNCATUS) [J].
EWALT, DR ;
PAYEUR, JB ;
MARTIN, BM ;
CUMMINS, DR ;
MILLER, WG .
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1994, 6 (04) :448-452
[10]   Rangiferine brucellosis on Baffin Island [J].
Ferguson, MAD .
JOURNAL OF WILDLIFE DISEASES, 1997, 33 (03) :536-543