Bacterial Isolation by Lectin-Modified Microengines

被引：279

作者：

Campuzano, Susana ^{[1
,2
]}

Orozco, Jahir ^{[1
]}

Kagan, Daniel ^{[1
]}

Guix, Maria ^{[1
,3
,4
]}

Gao, Wei ^{[1
]}

Sattayasamitsathit, Sirilak ^{[1
]}

Claussen, Jonathan C. ^{[1
]}

Merkoci, Arben ^{[3
,4
]}

Wang, Joseph ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Nanoengn, La Jolla, CA 92093 USA

[2] Univ Complutense Madrid, Dept Analyt Chem, E-28040 Madrid, Spain

[3] ICREA, E-08193 Barcelona, Spain

[4] Catalan Inst Nanotechnol, Nanobioelect & Biosensors Grp, E-08193 Barcelona, Spain

来源：

NANO LETTERS | 2012年 / 12卷 / 01期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

Nanomachines; microengines; lectin; E. coli isolation; theranostics; drug delivery; biodetection; complex samples; QUARTZ-CRYSTAL MICROBALANCE; SURFACE-PLASMON RESONANCE; ESCHERICHIA-COLI; CONCANAVALIN-A; PATHOGENIC BACTERIA; NONCULTURABLE STATE; RECOGNITION; BIOSENSOR; CARBOHYDRATE; QUANTIFICATION;

D O I：

10.1021/nl203717q

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

New template-based self-propelled gold/nickel/polyaniline/platinum (Au/Ni/PANI/Pt) microtubular engines, functionafized with the Concanavalin A (ConA) lectin bioreceptor, are shown to be extremely useful for the rapid, real-time isolation of Escherichia coli (E. coli) bacteria from fuel-enhanced environmental, food, and clinical samples. These multifunctional microtube engines combine the selective capture of E. coli with the uptake of polymeric drug-carrier particles to provide an attractive motion-based theranostics strategy. Triggered release of the captured bacteria is demonstrated by movement through a low-pH glycine-based dissociation solution. The smaller size of the new polymer-metal microengines offers convenient, direct, and label-free optical visualization of the captured bacteria and discrimination against nontarget cells.

引用

页码：396 / 401

页数：6

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