Myosin 1b promotes the formation of post-Golgi carriers by regulating actin assembly and membrane remodelling at the trans-Golgi network

被引：98

作者：

Almeida, Claudia G. ^{[1
,2
]}

Yamada, Ayako ^{[1
,3
]}

Tenza, Daniele ^{[1
,4
,5
]}

Louvard, Daniel ^{[1
,2
]}

Raposo, Graca ^{[1
,4
,5
]}

Coudrier, Evelyne ^{[1
,2
]}

机构：

[1] Inst Curie, Ctr Rech, F-75248 Paris, France

[2] Morphogenesis & Cell Signalizat CNRS, UMR144, F-75248 Paris, France

[3] CNRS, Cell & Tissue Imaging Facil, UMR 144, F-75248 Paris, France

[4] CNRS, UMR 168, F-75248 Paris, France

[5] Struct & Membrane Compartments CNRS, UMR144, F-75248 Paris, France

来源：

NATURE CELL BIOLOGY | 2011年 / 13卷 / 07期

关键词：

DROSOPHILA SPERMATID INDIVIDUALIZATION; LYSOSOMAL-ENZYMES; MOLECULAR MOTORS; LIVING CELLS; I-ALPHA; RECEPTOR; PROTEIN; ENDOSOMES; LOCALIZATION; TRAFFICKING;

D O I：

10.1038/ncb2262

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The function of organelles is intimately associated with rapid changes in membrane shape. By exerting force on membranes,the cytoskeleton and its associated motors have an important role in membrane remodelling. Actin and myosin 1 have been implicated in the invagination of the plasma membrane during endocytosis. However, whether myosin 1 and actin contribute to the membrane deformation that gives rise to the formation of post-Golgi carriers is unknown. Here we report that myosin 1b regulates the actin-dependent post-Golgi traffic of cargo, generates force that controls the assembly of F-actin foci and,together with the actin cytoskeleton, promotes the formation of tubules at the TGN. Our results provide evidence that actin and myosin 1 regulate organelle shape and uncover an important function for myosin 1b in the initiation of post-Golgi carrier formation by regulating actin assembly and remodelling TGN membranes.

引用

页码：779 / U424

页数：23

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