An analysis of avidin, biotin and their interaction at attomole levels by voltammetric and chromatographic techniques

被引:57
作者
Kizek, R [1 ]
Masarik, M
Kramer, KJ
Potesil, D
Bailey, M
Howard, JA
Klejdus, B
Mikelova, R
Adam, V
Trnkova, L
Jelen, F
机构
[1] Mendel Univ Brno, Dept Chem & Biochem, Zemedelska 1, Brno 61300, Czech Republic
[2] Acad Sci Czech Republ, Inst Biophys, Lab Biophys Chem & Mol Oncol, CS-61265 Brno, Czech Republic
[3] USDA ARS, Grain Mkt & Prod Res Ctr, Manhattan, KS 66502 USA
[4] Fac Sci, Dept Analyt Chem, Brno 61137, Czech Republic
[5] ProdiGene Inc, College Stn, TX 77845 USA
[6] Fac Sci, Dept Theoret & Phys Chem, Brno 61137, Czech Republic
关键词
avidin; biotin; avidin-biotin technology; square-wave voltammetry; modified electrode; high-performance liquid chromatography; diode array detection; electrochemical transfer technique; carbon paste electrode; drug analysis;
D O I
10.1007/s00216-004-3027-x
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The electroanalytical determination of avidin in solution, in a carbon paste, and in a transgenic maize extract was performed in acidic medium at a carbon paste electrode (CPE). The oxidative voltammetric signal resulting from the presence of tyrosine and tryptophan in avidin was observed using square-wave voltammetry. The process could be used to determine avidin concentrations up to 3 fM (100 amol in 3 mu l drop) in solution, 700 fM (174 fmol in 250 mu l solution) in an avidin-modified electrode, and 174 nM in a maize seed extract. In the case of the avidin-modified CPE, several parameters were studied in order to optimize the measurements, such as electrode accumulation time, composition of the avidin-modified CPE, and the elution time of avidin. In addition, the avidin-modified electrode was used to detect biotin in solution (the detection limit was 7.6 pmol in a 6 mu l drop) and to detect biotin in a pharmaceutical drug after various solvent extraction procedures. Comparable studies for the detection of biotin were developed using HPLC with diode array detection (HPLC- DAD) and flow injection analysis with electrochemical detection, which allowed biotin to be detected at levels as low as 614 pM and 6.6 nM, respectively. The effects of applied potential, acetonitrile content, and flow rate of the mobile phase on the FIA-ED signal were also studied.
引用
收藏
页码:1167 / 1178
页数:12
相关论文
共 77 条
[51]  
Prusa R, 2004, CLIN CHEM, V50, pA28
[52]   Determination of 3,5,6-trichloro-2-pyridinol (TCP) in water by a continuous competitive immunoassay system based on the streptavidin-biotin interaction [J].
Reyes, FD ;
Romero, JMF ;
de Castro, MDL .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2002, 372 (02) :366-372
[53]  
Reyes FD, 2001, ANAL CHIM ACTA, V436, P109, DOI 10.1016/S0003-2670(01)00899-6
[54]  
SILMAN RW, 1993, BIOTECHNOL ADV, V11, pIN7
[55]   DESIGN OF A GLUCOSE MINISENSOR BASED ON STREPTAVIDIN GLUCOSE-OXIDASE COMPLEX COUPLING WITH SELF-ASSEMBLED BIOTINYLATED PHOSPHOLIPID MEMBRANE ON SOLID SUPPORT [J].
SNEJDARKOVA, M ;
REHAK, M ;
OTTO, M .
ANALYTICAL CHEMISTRY, 1993, 65 (06) :665-668
[56]   ELECTROCHEMICAL DETERMINATION OF AVIDIN-BIOTIN BINDING USING AN ELECTROACTIVE BIOTIN DERIVATIVE AS A MARKER [J].
SUGAWARA, K ;
TANAKA, S ;
NAKAMURA, H .
BIOELECTROCHEMISTRY AND BIOENERGETICS, 1994, 33 (02) :205-207
[57]   Accumulation voltammetry of avidin and biotin using a biotin labeled with Nile Blue A [J].
Sugawara, K ;
Yamauchi, Y ;
Hoshi, S ;
Akatsuka, K ;
Yamamoto, F ;
Tanaka, S ;
Nakamura, H .
BIOELECTROCHEMISTRY AND BIOENERGETICS, 1996, 41 (02) :167-172
[58]   ELECTROCHEMICAL ASSAY OF AVIDIN AND BIOTIN USING A BIOTIN DERIVATIVE LABELED WITH AN ELECTROACTIVE COMPOUND [J].
SUGAWARA, K ;
TANAKA, S ;
NAKAMURA, H .
ANALYTICAL CHEMISTRY, 1995, 67 (02) :299-302
[59]   Electrochemical measurements of oligonucleotides in the presence of chromosomal DNA using membrane-covered carbon electrodes [J].
Wang, J ;
Cai, XH ;
Fernandes, JR ;
Grant, DH ;
Ozsoz, M .
ANALYTICAL CHEMISTRY, 1997, 69 (19) :4056-4059
[60]   Stripping analysis of nucleic acids at a heated carbon paste electrode [J].
Wang, J ;
Gründler, P ;
Flechsig, GU ;
Jasinski, M ;
Rivas, G ;
Sahlin, E ;
Paz, JLL .
ANALYTICAL CHEMISTRY, 2000, 72 (16) :3752-3756