Defined DNA/nanoparticle conjugates

被引：79

作者：

Ackerson, CJ ^{[1
]}

Sykes, MT ^{[1
]}

Kornberg, RD ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Biol Struct, Stanford, CA 94305 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2005年 / 102卷 / 38期

关键词：

DNA-DNA hybridization; monolayer-protected gold clusters; nanostructure;

D O I：

10.1073/pnas.0506290102

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Glutathione monolayer-protected gold clusters were reacted by place exchange with 19 or 20-residue thiolated oligonucleoticles. The resulting DNA/nanoparticle conjugates could be separated on the basis of the number of bound oligonucleoticles by gel electrophoresis and assembled with one another by DNA-DNA hybridization. This approach overcomes previous limitations of DNA/nanoparticle synthesis and yields conjugates that are precisely defined with respect to both gold and nucleic acid content.

引用

页码：13383 / 13385

页数：3

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