Ehrlichia ruminantium major antigenic protein gene (map1) variants are not geographically constrained and show no evidence of having evolved under positive selection pressure

被引:39
作者
Allsopp, MTEP [1 ]
Dorfling, CM
Maillard, JC
Bensaid, A
Haydon, DT
van Heerden, H
Allsopp, BA
机构
[1] Onderstepoort Vet Inst, Private Bag X5, ZA-0110 Onderstepoort, South Africa
[2] EMVT, CIRAD, F-34398 Montpellier 05, France
[3] EMVT, CIRAD, Pointe a Pitre, Guadeloupe, France
[4] Ctr Trop Vet Med, Roslin EH25 9RG, Midlothian, Scotland
关键词
D O I
10.1128/JCM.39.11.4200-4203.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In a search for tools to distinguish antigenic variants of Ehrlichia ruminantium, we sequenced the major antigenic protein genes (map1 genes) of 21 different isolates and found that the sequence polymorphisms were too great to permit the design of probes which could be used as markers for immunogenicity. Phylogenetic comparison of the 21 deduced MAP1 sequences plus another 9 sequences which had been previously published did not reveal any geographic clustering among the isolates. Maximum likelihood analysis of codon and amino acid changes over the phylogeny provided no statistical evidence that the gene is under positive selection pressure, suggesting that it may not be important for the evasion of host immune responses.
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收藏
页码:4200 / 4203
页数:4
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