Phenol biosensor based on Sonogel-Carbon transducer with tyrosinase alumina sol-gel immobilization

被引：51

作者：

Zejli, H. ^{[1
,2
,3
]}

de Cisneros, J. L. Hidalgo-Hidalgo ^{[3
]}

Naranjo-Rodriguez, I. ^{[3
]}

Liu, Baohong ^{[4
]}

Temsamani, K. R. ^{[2
]}

Marty, J. L. ^{[1
]}

机构：

[1] Univ Perpignan, Ctr Phytopharm, IMAGES EA 4218, F-66025 Perpignan, France

[2] Univ Abdelmalek Essaadi, Fac Sci Tetouan, Equipe Rech Electrochim & Syst Interfaciaux, Tetouan 93000, Morocco

[3] Univ Cadiz, Fac Sci, Dept Analyt Chem, Cadiz, Spain

[4] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2008年 / 612卷 / 02期

关键词：

biosensors; Sonogel-Carbon electrode; tyrosinase; alumina sol-gel; phenolic compounds;

D O I：

10.1016/j.aca.2008.02.029

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new biosensor for detection of phenols, based on tyrosinase immobilization with alumina sol-gel on Sonogel-Carbon transducer, has been developed. The electrode was prepared using high energy ultrasounds directly applied to the precursors. The alumina sol-gel provided a microenvironment for retaining the native structure and activity of the entrapped enzyme and a very low mass transport barrier to the enzyme substrates. Phenols are oxidized by tyrosinase biosensor to form a detectable product, which was determined at -300 mV vs. Ag/AgCl reference electrode. For phenol, the sensor exhibited a fast response which resulted from the porous structure and high enzyme loading of the sol-gel matrix. The linear range was from 5 x 10(-7) M to 3 x 10(-5) M, with a detection limit of 3 x 10(-7) M. The stability of the biosensor was also evaluated. (C) 2008 Published by Elsevier B.V.

引用

页码：198 / 203

页数：6

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