共 40 条
Transcripts synthesized by RNA polymerase III can be polyadenylated in an AAUAAA-dependent manner
被引:40
作者:
Borodulina, Olga R.
[1
]
Kramerov, Dmitri A.
[1
]
机构:
[1] Russian Acad Sci, Engelhardt Inst Mol Biol, Moscow 117984, Russia
来源:
基金:
俄罗斯基础研究基金会;
关键词:
RNA polymerase III;
polyadenylation signal;
transcriptional terminator;
poly(A);
SINEs of class T+;
B2;
RNA;
D O I:
10.1261/rna.1006608
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
It is well known that nearly all eukaryotic mRNAs contain a 3' poly(A) tail. A polyadenylation signal (AAUAAA) nearby the 3' end of pre-mRNA is required for poly(A) synthesis. The protein complex involved in the pre-mRNA polyadenylation is coupled with RNA polymerase If during the transcription of a gene. According to the commonly accepted view, only RNAs synthesized by RNA polymerase II can be polyadenylated in an AAUAAA-dependent manner. Here we report the polyadenylation of short interspersed elements (SINEs) B2 and VES transcripts generated by RNA polymerase III. HeLa cells were transfected with SINE constructs with or without polyadenylation signals. The analyses of the SINE transcripts showed that only the RNAs with the AAUAAA-signal contained poly(A) tails. Polyadenylated B2 RNA was found to be much more stable in cells than B2 RNA without a poly(A) tail.
引用
收藏
页码:1865 / 1873
页数:9
相关论文