Transcripts synthesized by RNA polymerase III can be polyadenylated in an AAUAAA-dependent manner

被引:40
作者
Borodulina, Olga R. [1 ]
Kramerov, Dmitri A. [1 ]
机构
[1] Russian Acad Sci, Engelhardt Inst Mol Biol, Moscow 117984, Russia
基金
俄罗斯基础研究基金会;
关键词
RNA polymerase III; polyadenylation signal; transcriptional terminator; poly(A); SINEs of class T+; B2; RNA;
D O I
10.1261/rna.1006608
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
It is well known that nearly all eukaryotic mRNAs contain a 3' poly(A) tail. A polyadenylation signal (AAUAAA) nearby the 3' end of pre-mRNA is required for poly(A) synthesis. The protein complex involved in the pre-mRNA polyadenylation is coupled with RNA polymerase If during the transcription of a gene. According to the commonly accepted view, only RNAs synthesized by RNA polymerase II can be polyadenylated in an AAUAAA-dependent manner. Here we report the polyadenylation of short interspersed elements (SINEs) B2 and VES transcripts generated by RNA polymerase III. HeLa cells were transfected with SINE constructs with or without polyadenylation signals. The analyses of the SINE transcripts showed that only the RNAs with the AAUAAA-signal contained poly(A) tails. Polyadenylated B2 RNA was found to be much more stable in cells than B2 RNA without a poly(A) tail.
引用
收藏
页码:1865 / 1873
页数:9
相关论文
共 40 条
[21]   THE SEQUENCES HOMOLOGOUS TO MAJOR INTERSPERSED REPEATS B1 AND B2 OF MOUSE GENOME ARE PRESENT IN MESSENGER-RNA AND SMALL CYTOPLASMIC POLY(A)+RNA [J].
KRAMEROV, DA ;
LEKAKH, IV ;
SAMARINA, OP ;
RYSKOV, AP .
NUCLEIC ACIDS RESEARCH, 1982, 10 (23) :7477-7491
[22]   Transcription of mammalian messenger RNAs by a nuclear RNA polymerase of mitochondrial origin [J].
Kravchenko, JE ;
Rogozin, IB ;
Koonin, EV ;
Chumakov, PM .
NATURE, 2005, 436 (7051) :735-739
[23]   UBIQUITOUS TRANSPOSON-LIKE REPEATS B1 AND B2 OF THE MOUSE GENOME - B2 SEQUENCING [J].
KRAYEV, AS ;
MARKUSHEVA, TV ;
KRAMEROV, DA ;
RYSKOV, AP ;
SKRYABIN, KG ;
BAYEV, AA ;
GEORGIEV, GP .
NUCLEIC ACIDS RESEARCH, 1982, 10 (23) :7461-7475
[24]   A family of poly(U) polymerases [J].
Kwak, Jae Eun ;
Wickens, Marvin .
RNA, 2007, 13 (06) :860-867
[25]   POLYADENYLYLATION OF AN MESSENGER-RNA PRECURSOR OCCURS INDEPENDENTLY OF TRANSCRIPTION BY RNA POLYMERASE-II INVIVO [J].
LEWIS, ED ;
MANLEY, JL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (22) :8555-8559
[26]   A COMPLEX PROTEIN ASSEMBLY CATALYZES POLYADENYLATION OF MESSENGER-RNA PRECURSORS [J].
MANLEY, JL .
CURRENT OPINION IN GENETICS & DEVELOPMENT, 1995, 5 (02) :222-228
[27]   Predicting mammalian SINE subfamily activity from A-tail length [J].
Odom, GL ;
Robichaux, JL ;
Deininger, PL .
MOLECULAR BIOLOGY AND EVOLUTION, 2004, 21 (11) :2140-2148
[28]   SINEs and LINEs: symbionts of eukaryotic genomes with a common tail [J].
Ohshima, K ;
Okada, N .
CYTOGENETIC AND GENOME RESEARCH, 2005, 110 (1-4) :475-490
[29]   Emerging themes in non-coding RNA quality control [J].
Reinisch, Karin M. ;
Wolin, Sandra L. .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 2007, 17 (02) :209-214
[30]   Human retroelements may introduce intragenic polyadenylation signals [J].
Roy-Engel, AM ;
El-Sawy, M ;
Farooq, L ;
Odom, GL ;
Perepelitsa-Belancio, V ;
Bruch, H ;
Oyeniran, OO ;
Deininger, PL .
CYTOGENETIC AND GENOME RESEARCH, 2005, 110 (1-4) :365-371