Mammalian miRNA RISC Recruits CAF1 and PABP to Affect PABP-Dependent Deadenylation

被引：297

作者：

Fabian, Marc R. ^{[1
,2
]}

Mathonnet, Geraldine ^{[1
,2
]}

Sundermeier, Thomas ^{[1
,2
]}

Mathys, Hansruedi ^{[3
]}

Zipprich, Jakob T. ^{[3
]}

Svitkin, Yuri V. ^{[1
,2
]}

Rivas, Fabiola ^{[4
,5
]}

Jinek, Martin ^{[6
]}

Wohischlegel, James ^{[7
]}

Doudna, Jennifer A. ^{[6
]}

Chen, Chyi-Ying A. ^{[8
]}

Shyu, Ann-Bin ^{[8
]}

Yates, John R., III ^{[7
]}

Hannon, Gregory J. ^{[4
,5
]}

Filipowicz, Witold ^{[3
]}

Duchaine, Thomas F. ^{[1
,2
]}

Sonenberg, Nahum ^{[1
,2
]}

机构：

[1] McGill Univ, Dept Biochem, Montreal, PQ H3G 1Y6, Canada

[2] McGill Univ, Goodman Canc Ctr, Montreal, PQ H3G 1Y6, Canada

[3] Friedrich Miescher Inst Biomed Res, CH-4002 Basel, Switzerland

[4] Cold Spring Harbor Lab, Howard Hughes Med Inst, Cold Spring Harbor, NY 11724 USA

[5] Cold Spring Harbor Lab, Watson Sch Biol Sci, Cold Spring Harbor, NY 11724 USA

[6] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[7] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

[8] Univ Texas Med Sch, Dept Biochem & Mol Biol, Houston, TX 77030 USA

来源：

MOLECULAR CELL | 2009年 / 35卷 / 06期

关键词：

MESSENGER-RNA DEADENYLATION; PROTEIN IDENTIFICATION TECHNOLOGY; TRANSLATION INITIATION-FACTOR; POLY(A) BINDING-PROTEIN; 3' UNTRANSLATED REGION; POLY(A)-BINDING PROTEIN; HUMAN-CELLS; GW182; PROTEINS; IN-VITRO; SACCHAROMYCES-CEREVISIAE;

D O I：

10.1016/j.molcel.2009.08.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNAs (miRNAs) inhibit mRNA expression in general by base pairing to the 3'UTR of target mRNAs and consequently inhibiting translation and/or initiating poly(A) tail deadenylation and mRNA destabilization. Here we examine the mechanism and kinetics of miRNA-mediated deadenylation in mouse Krebs-2 ascites extract. We demonstrate that miRNA-mediated mRNA deadenylation occurs subsequent to initial translational inhibition, indicating a two-step mechanism of miRNA action, which serves to consolidate repression. We show that a let-7 miRNA-loaded RNA-induced silencing complex (miRISC) interacts with the poly(A)-binding protein (PABP) and the CAF1 and CCR4 deadenylases. In addition, we demonstrate that miRNA-mediated deadenylation is dependent upon CAF1 activity and PABP, which serves as a bona fide miRNA coactivator. Importantly, we present evidence that GW182, a core component of the miRISC, directly interacts with PABP via its C-terminal region and that this interaction is required for miRNA-mediated deadenylation.

引用

页码：868 / 880

页数：13

共 67 条

[1] MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].

Bartel, David P. .

CELL, 2007, 131 (04) :11-29

[2] MRNA degradation by miRNAs and GW182 requires both CCR4:NOT deadenylase and DCP1:DCP2 decapping complexes [J].

Behm-Ansmant, Isabelle ;

Rehwinkel, Jan ;

Doerks, Tobias ;

Stark, Alexander ;

Bork, Peer ;

Izaurralde, Elisa .

GENES & DEVELOPMENT, 2006, 20 (14) :1885-1898

[3] Conservation of the deadenylase activity of proteins of the Caf1 family in human [J].

Bianchin, C ;

Mauxion, F ;

Sentis, S ;

Séraphin, B ;

Corbo, L .

RNA, 2005, 11 (04) :487-494

[4] Functional characterization of IRESes by an inhibitor of the RNA helicase eIF4A [J].

Bordeleau, ME ;

Mori, A ;

Oberer, M ;

Lindqvist, L ;

Chard, LS ;

Higa, T ;

Belsham, GJ ;

Wagner, G ;

Tanaka, J ;

Pelletier, J .

NATURE CHEMICAL BIOLOGY, 2006, 2 (04) :213-220

[5] Multiple independent domains of dGW182 function in miRNA-mediated repression in Drosophila [J].

Chekulaeva, Marina ;

Filipowicz, Witold ;

Parker, Roy .

RNA, 2009, 15 (05) :794-803

[6]

Chendrimada TP, 2007, NATURE, V447, P823, DOI 10.1038/nature05841

[7] TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing [J].

Chendrimada, TP ;

Gregory, RI ;

Kumaraswamy, E ;

Norman, J ;

Cooch, N ;

Nishikura, K ;

Shiekhattar, R .

NATURE, 2005, 436 (7051) :740-744

[8] GW182 family proteins are crucial for microRNA-mediated gene silencing [J].

Ding, Lei ;

Han, Min .

TRENDS IN CELL BIOLOGY, 2007, 17 (08) :411-416

[9] Repression of C-elegans microRNA targets at the initiation level of translation requires GW182 proteins [J].

Ding, Xavier C. ;

Grosshans, Helge .

EMBO JOURNAL, 2009, 28 (03) :213-222

[10] GW182 interaction with Argonaute is essential for miRNA-mediated translational repression and mRNA decay [J].

Eulalio, Ana ;

Huntzinger, Eric ;

Izaurralde, Elisa .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2008, 15 (04) :346-353

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