Heterologous expression in Saccharopolyspora erythraea of a pentaketide synthase derived from the spinosyn polyketide synthase

被引：31

作者：

Martin, CJ

Timoney, MC

Sheridan, RM

Kendrew, SG

Wilkinson, B

Staunton, J

Leadlay, PF

机构：

[1] Biot Technol Ltd, Saffron Walden CB10 1XL, Essex, England

[2] Univ Cambridge, Cambridge Ctr Mol Recognit, Cambridge CB2 1GA, England

[3] Univ Cambridge, Dept Biochem, Cambridge CB2 1GA, England

[4] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

[5] Univ Cambridge, Cambridge Ctr Mol Recognit, Cambridge CB2 1EW, England

[6] Pfizer Global Pharmaceut, Tadworth KT20 7NS, England

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2003年 / 1卷 / 23期

关键词：

D O I：

10.1039/b310740j

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A truncated version of the spinosyn polyketide synthase comprising the loading module and the first four extension modules fused to the erythromycin thioesterase domain was expressed in Saccharopolyspora erythraea. A novel pentaketide lactone product was isolated, identifying cryptic steps of spinosyn biosynthesis and indicating the potential of this approach for the biosynthetic engineering of spinosyn analogues. A pathway for the formation of the tetracyclic spinosyn aglycone is proposed.

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收藏

页码：4144 / 4147

页数：4

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