Duodenal Enteroendocrine I-Cells Contain mRNA Transcripts Encoding Key Endocannabinoid and Fatty Acid Receptors

被引:95
作者
Sykaras, Alexandros G. [1 ,2 ]
Demenis, Claire [1 ]
Case, R. Maynard [1 ]
McLaughlin, John T. [3 ]
Smith, Craig P. [1 ]
机构
[1] Univ Manchester, Fac Life Sci, Manchester, Lancs, England
[2] Univ Crete, Fac Med, Grad Programme Mol Basis Human Dis, Iraklion, Crete, Greece
[3] Univ Manchester, Sch Med, Sch Translat Med, Manchester, Lancs, England
来源
PLOS ONE | 2012年 / 7卷 / 08期
基金
英国生物技术与生命科学研究理事会;
关键词
PROTEIN-COUPLED RECEPTOR; GLUCAGON-LIKE PEPTIDE-1; VAGAL AFFERENT NEURONS; CHOLECYSTOKININ SECRETION; GLYCEMIC CONTROL; ENDOCRINE CELL; EXPRESSION; GUT; RELEASE; GPR119;
D O I
10.1371/journal.pone.0042373
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Enteroendocrine cells have a critical role in regulation of appetite and energy balance. I-cells are a subtype of enteroendocrine cells localized in duodenum that release cholecystokinin in response to ingested fat and amino-acids. Despite their potentially pivotal role in nutrient sensing and feeding behaviour, native I-cells have previously been difficult to isolate and study. Here we describe a robust protocol for the isolation and characterization of native duodenal I-cells and additionally, using semi-quantitative RT-PCR we determined that mouse duodenal I-cells contain mRNA transcripts encoding key fatty acid and endocannabinoid receptors including the long chain fatty acid receptors GPR40/FFAR1, GPR120/O3FAR1; short chain fatty acid receptors GPR41/FFAR3 and GPR43/FFAR2; the oleoylethanolamide receptor GPR119 and the classic endocannabinoid receptor CB1. These data suggest that I-cells sense a wide range of gut lumen nutrients and also have the capacity to respond to signals of fatty-acid derivatives or endocannabinoid peptides.
引用
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页数:10
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[1]   The orphan G protein-coupled receptor GPR40 is activated by medium and long chain fatty acids [J].
Briscoe, CP ;
Tadayyon, M ;
Andrews, JL ;
Benson, WG ;
Chambers, JK ;
Eilert, MM ;
Ellis, C ;
Elshourbagy, NA ;
Goetz, AS ;
Minnick, DT ;
Murdock, PR ;
Sauls, HR ;
Shabon, U ;
Spinage, LD ;
Strum, JC ;
Szekeres, PG ;
Tan, KB ;
Way, JM ;
Ignar, DM ;
Wilson, S ;
Muir, AI .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (13) :11303-11311
[2]   The orphan G protein-coupled receptors GPR41 and GPR43 are activated by propionate and other short chain carboxylic acids [J].
Brown, AJ ;
Goldsworthy, SM ;
Barnes, AA ;
Eilert, MM ;
Tcheang, L ;
Daniels, D ;
Muir, AI ;
Wigglesworth, MJ ;
Kinghorn, I ;
Fraser, NJ ;
Pike, NB ;
Strum, JC ;
Steplewski, KM ;
Murdock, PR ;
Holder, JC ;
Marshall, FH ;
Szekeres, PG ;
Wilson, S ;
Ignar, DM ;
Foord, SM ;
Wise, A ;
Dowell, SJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (13) :11312-11319
[3]   ELECTRON IMMUNOHISTOCHEMICAL EVIDENCE FOR HUMAN INTESTINAL I CELL AS SOURCE OF CCK [J].
BUCHAN, AMJ ;
POLAK, JM ;
SOLCIA, E ;
CAPELLA, C ;
HUDSON, D ;
PEARSE, AGE .
GUT, 1978, 19 (05) :403-407
[4]   Expression of cannabinoid CB1 receptors by vagal afferent neurons is inhibited by cholecystokinin [J].
Burdyga, G ;
Lal, S ;
Varro, A ;
Dimaline, R ;
Thompson, DG ;
Dockray, GJ .
JOURNAL OF NEUROSCIENCE, 2004, 24 (11) :2708-2715
[5]   Ghrelin receptors in rat and human nodose ganglia: putative role in regulating CB-1 and MCH receptor abundance [J].
Burdyga, Galina ;
Varro, Andrea ;
Dimaline, Rod ;
Thompson, David G. ;
Dockray, Graham J. .
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2006, 290 (06) :G1289-G1297
[6]  
Chandra Rashmi, 2007, Curr Opin Endocrinol Diabetes Obes, V14, P63, DOI 10.1097/MED.0b013e3280122850
[7]   LOCALIZATION OF MUCIN (MUC2 AND MUC3) MESSENGER-RNA AND PEPTIDE EXPRESSION IN HUMAN NORMAL INTESTINE AND COLON-CANCER [J].
CHANG, SK ;
DOHRMAN, AF ;
BASBAUM, CB ;
HO, SB ;
TSUDA, T ;
TORIBARA, NW ;
GUM, JR ;
KIM, YS .
GASTROENTEROLOGY, 1994, 107 (01) :28-36
[8]   A role for intestinal endocrine cell-expressed g protein-coupled receptor 119 in glycemic control by enhancing glucagon-like peptide-1 and glucose-dependent insulinotropic peptide release [J].
Chu, Zhi-Liang ;
Carroll, Chris ;
Alfonso, Jean ;
Gutierrez, Veronica ;
He, Hongmei ;
Lucman, Annette ;
Pedraza, Melinda ;
Mondala, Helen ;
Gao, Hui ;
Bagnol, Didier ;
Chen, Ruoping ;
Jones, Robert M. ;
Behan, Dominic P. ;
Leonard, James .
ENDOCRINOLOGY, 2008, 149 (05) :2038-2047
[9]   A role for β-cell-expressed G protein-coupled receptor 119 in glycemic control by enhancing glucose-dependent insulin release [J].
Chu, Zhi-Liang ;
Jones, Robert M. ;
He, Hongmei ;
Carroll, Chris ;
Gutierrez, Veronica ;
Lucman, Annette ;
Moloney, Molly ;
Gao, Hui ;
Mondala, Helen ;
Bagnol, Didier ;
Unett, David ;
Liang, Yin ;
Demarest, Keith ;
Semple, Graeme ;
Behan, Dominic P. ;
Leonard, James .
ENDOCRINOLOGY, 2007, 148 (06) :2601-2609
[10]   Overactivity of the intestinal endocannabinoid system in celiac disease and in methotrexate-treated rats [J].
D'Argenio, Giuseppe ;
Petrosino, Stefania ;
Gianfrani, Carmen ;
Valenti, Marta ;
Scaglione, Giuseppe ;
Grandone, Ilenia ;
Nigam, Santosh ;
Sorrentini, Italo ;
Mazzarella, Giuseppe ;
Di Marzo, Vincenzo .
JOURNAL OF MOLECULAR MEDICINE-JMM, 2007, 85 (05) :523-530