The heme monooxygenase cytochrome P450cam can be engineered to oxidize ethane to ethanol

被引：91

作者：

Xu, F

Bell, SG

Lednik, J

Insley, A

Rao, ZH ^{[1
]}

Wong, LL

机构：

[1] Tsinghua Univ, Struct Biol Lab, Sch Life Sci & Engn, Beijing 100084, Peoples R China

[2] Inst Biophys, Beijing 100101, Peoples R China

[3] Univ Oxford, Inorgan Chem Lab, Dept Chem, Oxford OX1 3QR, England

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 26期

关键词：

alkane oxidation; heme proteins; iron; metalloenzymes; protein engineering;

D O I：

10.1002/anie.200462630

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Graph Presented) A NADH turnover rate of 741 min-1 in the oxidition of ethane to ethanol is observed with an engineered form of the heme monooxygenase cytochrome P450cam - the first example of such activity for a P450 enzyme (CC analysis shown). Ethanol is formed at 78 min-1 (10.5 % coupling). The mutant is ≈45 % high-spin in the absence of substrate, making it a useful platform for P450 structure-function studies. © 2005 Wiley-VCH Verlag GmbH &. Co. KGaA.

引用

页码：4029 / 4032

页数：4

共 19 条

[1] Butane and propane oxidation by engineered cytochrome P450cam [J].