Efficient one-step synthesis of biologically related lariat RNAs by a deoxyribozyme

被引：37

作者：

Wang, YM ^{[1
]}

Silverman, SK ^{[1
]}

机构：

[1] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 36期

关键词：

deoxyribozymes; DNA; enzyme catalysis; lariat RNA; macrocycles;

D O I：

10.1002/anie.200501643

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Closing the loop: The artificial 6BX22 deoxyribozyme (in green) synthesizes naturally occurring lariat RNAs in one step from readily available linear RNA substrates. The exquisite site selectivity of this macrocyclization reaction - which can form a 1597-membered ring in 72% yield with < 0.1 % side products - highlights the utility of catalytic DNA for transformations that are not possible with traditional synthetic methods. (Chemical Equation Presented). © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

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收藏

页码：5863 / 5866

页数：4

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