Promiscuous, non-catalytic, tandem carbohydrate-binding modules modulate the cell-wall structure and development of transgenic tobacco (Nicotiana tabacum) plants

被引:26
作者
Obembe, Olawole O.
Jacobsen, Evert
Timmers, Jaap
Gilbert, Harry
Blake, Anthony W.
Knox, J. Paul
Visser, Richard G. F.
Vincken, Jean-Paul
机构
[1] Wageningen Univ, Lab Plant Breeding, Grad Sch Expt Plant Sci, NL-6700 AJ Wageningen, Netherlands
[2] Newcastle Univ, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England
[3] Univ Leeds, Ctr Plant Sci, Leeds LS2 9JT, W Yorkshire, England
关键词
carbohydrate-binding modules; cell expansion; cell wall modification; cellulose-hemicellulose networks; plant growth and development; promiscuous polysaccharide recognition;
D O I
10.1007/s10265-007-0099-7
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We have compared heterologous expression of two types of carbohydrate binding module (CBM) in tobacco cell walls. These are the promiscuous CBM29 modules (a tandem CBM29-1-2 and its single derivative CBM29-2), derived from a non-catalytic protein1, NCP1, of the Piromyces equi cellulase/ hemicellulase complex, and the less promiscuous tandem CBM2 (b) under bar -1-2 from the Cellulomonas fimi xylanase 11A. CBM-labelling studies revealed that CBM29-1-2 binds indiscriminately to every tissue of the wild-type tobacco stem whereas binding of CBM2 (b) under bar -1-2 was restricted to vascular tissue. The promiscuous CBM29-1-2 had much more pronounced effects on transgenic tobacco plants than the less promiscuous CBM2 (b) under bar -1-2. Reduced stem elongation and prolonged juvenility, resulting in delayed flower development, were observed in transformants expressing CBM29-1-2 whereas such growth phenotypes were not observed for CBM2 (b) under bar -1-2 plants. Histological examination and electron microscopy revealed layers of collapsed cortical cells in the stems of CBM29-1-2 plants whereas cellular deformation in the stem cortical cells of CBM2 (b) under bar -1-2 transformants was less severe. Altered cell expansion was also observed in most parts of the CBM29-1-2 stem whereas for the CBM2 (b) under bar -1-2 stem this was observed in the xylem cells only. The cellulose content of the transgenic plants was not altered. These results support the hypothesis that CBMs can modify cell wall structure leading to modulation of wall loosening and plant growth.
引用
收藏
页码:605 / 617
页数:13
相关论文
共 49 条
[21]   Differential recognition of plant cell walls by microbial xylan-specific carbohydrate-binding modules [J].
McCartney, L ;
Blake, AW ;
Flint, J ;
Bolam, DN ;
Boraston, AB ;
Gilbert, HJ ;
Knox, JP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (12) :4765-4770
[22]   Glycoside hydrolase carbohydrate-binding modules as molecular probes for the analysis of plant cell wall polymers [J].
McCartney, L ;
Gilbert, HJ ;
Bolam, DN ;
Boraston, AB ;
Knox, JP .
ANALYTICAL BIOCHEMISTRY, 2004, 326 (01) :49-54
[23]   The galactose residues of xyloglucan are essential to maintain mechanical strength of the primary cell walls in Arabidopsis during growth [J].
Peña, MJ ;
Ryden, P ;
Madson, M ;
Smith, AC ;
Carpita, NC .
PLANT PHYSIOLOGY, 2004, 134 (01) :443-451
[24]   The history of tissue tension [J].
Peters, WS ;
Tomos, AD .
ANNALS OF BOTANY, 1996, 77 (06) :657-665
[25]   Local expression of expansin induces the entire process of leaf development and modifies leaf shape [J].
Pien, S ;
Wyrzykowska, J ;
McQueen-Mason, S ;
Smart, C ;
Fleming, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (20) :11812-11817
[26]  
QUENTIN MGE, 2003, THESIS WAGENINGEN U
[27]   Tensile properties of Arabidopsis cell walls depend on both a xyloglucan cross-linked microfibrillar network and rhamnogalacturonan II-borate complexes [J].
Ryden, P ;
Sugimoto-Shirasu, K ;
Smith, AC ;
Findlay, K ;
Reiter, WD ;
McCann, MC .
PLANT PHYSIOLOGY, 2003, 132 (02) :1033-1040
[28]   Growth modulation of transgenic potato plants by heterologous expression of bacterial carbohydrate-binding module [J].
Safra-Dassa, Liat ;
Shani, Ziv ;
Danin, Ahihud ;
Roiz, Levava ;
Shoseyov, Oded ;
Wolf, Shmuel .
MOLECULAR BREEDING, 2006, 17 (04) :355-364
[29]   Mannan transglycosylase:: a novel enzyme activity in cell walls of higher plants [J].
Schröder, R ;
Wegrzyn, TF ;
Bolitho, KM ;
Redgwell, RJ .
PLANTA, 2004, 219 (04) :590-600
[30]  
Shoseyov O, 2001, US Patent, Patent No. [6,184,440, 6184440]