N-acetyl-D-galactosamine-specific lectins from Phaeolepiota aurea

被引：12

作者：

Kawagishi, H ^{[1
]}

Wasa, T ^{[1
]}

Murata, T ^{[1
]}

Usui, T ^{[1
]}

Kimura, A ^{[1
]}

Chiba, S ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC AGR,DEPT APPL BIOSCI,SAPPORO,HOKKAIDO 060,JAPAN

来源：

PHYTOCHEMISTRY | 1996年 / 41卷 / 04期

关键词：

Phaeolepiota aurea; Agaricaceae; mushroom; N-D-acetylgalactosamine-specific lectins; PAL-I; PAL-II;

D O I：

10.1016/0031-9422(95)00733-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two lectins, PAL-I and PAL-II, were isolated from the mushroom Phaeolepiota aurea by affinity chromatography on acid-treated Sepharose CL-4B followed by reverse-phase FPLC on ProRPC. Both of the lectins were tetramers of 16 kDa subunits. The lectins had little agglutination activity against native erythrocytes but Pronase treatment of erythrocytes increased the sensitivity to agglutination by the lectins. Both lectins exhibited slight preferences for type A compared with type B and O erythrocytes. In haemagglutination inhibition assays, N-acetylgalactosamine and both anomers of methyl N-acetylgalactosaminide were the best inhibitors.

引用

页码：1013 / 1016

页数：4

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