Development and use of an internal positive control for detection of Bordetella pertussis by PCR

被引：8

作者：

Herwegh, S

Carnoy, C

Wallet, F

Loïez, C

Courcol, RJ ^{[1
]}

机构：

[1] Hop A Calmette, Lab Bacteriol Hyg, F-57037 Lille, France

[2] Inst Biol Lille, INSERM E0364, F-59021 Lille, France

[3] Fac Sci Pharmaceut & Biol, Lille, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 05期

关键词：

D O I：

10.1128/JCM.43.5.2462-2464.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An internal control of amplification was constructed by recombinant PCR to detect PCR inhibitors. This exogenous DNA was included in the reaction mixture and coamplified with the target gene. This detection was successfully applied to the diagnosis of whooping cough by amplification of a fragment of Bordetella pertussis IS481.

引用

收藏

页码：2462 / 2464

页数：3

相关论文

共 20 条

[1] A 2ND CHROMOSOMAL GENE NECESSARY FOR INTIMATE ATTACHMENT OF ENTEROPATHOGENIC ESCHERICHIA-COLI TO EPITHELIAL-CELLS [J].

DONNENBERG, MS ;

YU, J ;

KAPER, JB .

JOURNAL OF BACTERIOLOGY, 1993, 175 (15) :4670-4680

[2] Inhibition of PCR by agar from bacteriological transport media [J].

Gibb, AP ;

Wong, S .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (01) :275-276

[3] SITE-DIRECTED MUTAGENESIS BY OVERLAP EXTENSION USING THE POLYMERASE CHAIN-REACTION [J].

HO, SN ;

HUNT, HD ;

HORTON, RM ;

PULLEN, JK ;

PEASE, LR .

GENE, 1989, 77 (01) :51-59

[4] Practical considerations in design of internal amplification controls for diagnostic PCR assays [J].

Hoorfar, J ;

Malorny, B ;

Abdulmawjood, A ;

Cook, N ;

Wagner, M ;

Fach, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (05) :1863-1868

[5] Making internal amplification control mandatory for diagnostic PCR [J].

Hoorfar, J ;

Cook, N ;

Malorny, B ;

Wagner, M ;

De Medici, D ;

Abdulmaajood, A ;

Fach, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (12) :5835-5835

[6] Rapid detection of Chlamydia pneumoniae by PCR-enzyme immunoassay [J].

Jantos, CA ;

Roggendorf, R ;

Wuppermann, FN ;

Hegemann, JH .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (07) :1890-1894

[7] MYCOBACTERIUM-SMEGMATIS STRAIN FOR DETECTION OF MYCOBACTERIUM-TUBERCULOSIS BY PCR USED AS INTERNAL CONTROL FOR INHIBITION OF AMPLIFICATION AND FOR QUANTIFICATION OF BACTERIA [J].

KOLK, AHJ ;

NOORDHOEK, GT ;

DELEEUW, O ;

KUIJPER, S ;

VANEMBDEN, JDA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (05) :1354-1356

[8] Evaluation of PCR for diagnosis of Bordetella pertussis and Bordetella parapertussis infections [J].

Lind-Brandberg, L ;

Welinder-Olsson, C ;

Lagergård, T ;

Taranger, J ;

Trollfors, B ;

Zackrisson, G .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (03) :679-683

[9] Species-specific and ubiquitous-DNA-based assays for rapid identification of Staphylococcus aureus [J].

Martineau, F ;

Picard, FJ ;

Roy, PH ;

Ouellete, M ;

Bergeron, MG .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (03) :618-623

[10] PCR AND IN-VITRO CULTIVATION FOR DETECTION OF LEISHMANIA SPP IN DIAGNOSTIC SAMPLES FROM HUMANS AND DOGS [J].

MATHIS, A ;

DEPLAZES, P .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (05) :1145-1149