Effects of constituents from the bark of Magnolia obovata on nitric oxide production in lipopolysaccharide-activated macrophages

被引：108

作者：

Matsuda, H ^{[1
]}

Kageura, T ^{[1
]}

Oda, M ^{[1
]}

Morikawa, T ^{[1
]}

Sakamoto, Y ^{[1
]}

Yoshikawa, M ^{[1
]}

机构：

[1] Kyoto Pharmaceut Univ, Yamashima Ku, Kyoto 6078412, Japan

来源：

CHEMICAL & PHARMACEUTICAL BULLETIN | 2001年 / 49卷 / 06期

关键词：

Magnolia obovata; neolignan; nitric oxide; inducible nitric oxide synthase; nuclear factor-kappa B; inhibitor;

D O I：

10.1248/cpb.49.716

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The methanolic extract from a Japanese herbal medicine, the bark of Magnolia obovata, was found to inhibit nitric oxide (NO) production in lipopolysaccharide (LPS)-activated macrophages. By bioassay-guided separation, three neolignans (magnolol, honokiol, obovatol) and three sesquiterpenes (alpha-eudesmol, beta-eudesmol, gamma-eudesmol) were obtained as active constituents. A trineolignan (magnolianin), a phenylpropanoid glycoside (syringin), lignan glycosides (liriodendrin, (+)-syringaresinol 4'-O-beta -D-glucopyranoside) and a sesquiterpene (caryophyllene oxide) did not show any activity. On the other hand, sesquiterpene-neolignans (eudesmagnolol, ciovanemagnolol, caryolanemagnolol, eudeshonokiol A, eudesobovatol A) showed the strong cytotoxic effects. Active constituents (magnolol, honokiol, obovatol) showed weak inhibition for inducible NO synthase (INOS) enzyme activity, but potent inhibition of iNOS induction and activation of nuclear factor-kappaB.

引用

页码：716 / 720

页数：5

共 48 条

[1]

Bae FA, 1998, BIOL PHARM BULL, V21, P990, DOI 10.1248/bpb.21.990

[2]

Beek T. A. V., 1989, PHYTOCHEMISTRY, V28, P1909

[3]

DEYAMA T, 1983, CHEM PHARM BULL, V31, P2993

[4]

DING AH, 1988, J IMMUNOL, V141, P2407