A FRET-based fluorogenic phosphine for live-cell Imaging with the Staudinger ligation

被引：155

作者：

Hangauer, Matthew J.

Bertozzi, Carolyn R. ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 13期

关键词：

azides; fluorescent probes; glycosylation; phosphines; Staudinger ligation;

D O I：

10.1002/anie.200704847

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Azide imaging: A fluorogenic phosphine based on a FRET-quenching mechanism allows for live-cell imaging of azido sugars by the Staudinger ligation. This design strategy can accommodate numerous fluorophores and complementary quenchers, enabling extension to multi-color imaging. In the image shown, the nuclei are blue while the cell surfaces and Golgi are green. (Figure Presented). © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：2394 / 2397

页数：4

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