Enhancing the production of cinnamyl glycosides in compact callus aggregate cultures of Rhodiola rosea by biotransformation of cinnamyl alcohol

被引：35

作者：

György, Z

Tolonen, A

Pakonen, M

Neubauer, P

Hohtola, A

机构：

[1] Univ Oulu, Dept Proc & Environm Engn, Bioproc Engn Lab, Oulu 90014, Finland

[2] Univ Oulu, Dept Chem, Oulu 90014, Finland

[3] Univ Oulu, Dept Biol, Oulu 90014, Finland

来源：

PLANT SCIENCE | 2004年 / 166卷 / 01期

基金：

芬兰科学院;

关键词：

Rhodiola rosea; cinnamyl alcohol; cinnamyl glycosides; compact callus aggregates;

D O I：

10.1016/j.plantsci.2003.09.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Compact callus aggregates (CCA) of Rhodiola rosea were established. The growth curve of CCA culture was determined. No secondary compounds are produced in callus. Cinnamyl alcohol was added to the media in several concentrations between 0.05 and 5 mM in order to stimulate the production of cinnamyl glycosides. The optimal concentration of the precursor was determined. The consumption of cinnamyl alcohol and the production of cinnamyl glycosides were followed by daily sampling. The chemical analyses of the samples were performed by HPLC/MS. Among the cinnamyl glycosides rosin was produced in high amounts. A three to sixfold rosin content was achieved in the treated calli comparing to the content of wild growing plants. Rosavin was produced in traces only. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

引用

页码：229 / 236

页数：8

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