Gas chromatography and high-performance liquid chromatography of natural steroids

被引：146

作者：

Shimada, K ^{[1
]}

Mitamura, K ^{[1
]}

Higashi, T ^{[1
]}

机构：

[1] Kanazawa Univ, Fac Pharmaceut Sci, Kanazawa, Ishikawa 9200934, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 935卷 / 1-2期

关键词：

reviews; steroids; hormones; androgens; corticoids; estrogens; sterols; bile acids; vitamin D; phytosteroids;

D O I：

10.1016/S0021-9673(01)00943-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This review article underlines the importance of gas chromatography (GC), high-performance liquid chromatography (HPLC) and their hyphenated techniques using mass spectrometry (MS) for the determination of natural steroids, especially in human biological fluids. Steroids are divided into eight categories based on their structures and functions, and recent references using the above methodologies for the analysis of these steroids are cited. GC and GC-MS are commonly used for the determination of volatile steroids. Although HPLC is a widely used analytical method for the determination of steroids including the conjugated type in biological fluids, LC-MS is considered to be the most promising one for this purpose because of its sensitivity, specificity and versatility. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：141 / 172

页数：32

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