共 41 条
MCM-41 mesoporous material modified carbon paste electrode for the determination of cardiac troponin I by anodic stripping voltammetry
被引:73
作者:
Guo, HS
He, NY
[1
]
Ge, SX
Yang, D
Zhang, JN
机构:
[1] SE Univ, State Key Lab Bioelect, Chien Shiung Wu Lab, Nanjing 210096, Peoples R China
[2] Nanjing Med Univ, Inst Cardiovasc Dis, Nanjing 210029, Peoples R China
[3] Zhuzhou Inst Technol, Lab NanoBiomed & Devices, Zhuzhou 412008, Peoples R China
来源:
基金:
中国国家自然科学基金;
关键词:
cardiac troponin I;
mesoporous materials;
MCM-4;
1;
carbon paste electrode;
anodic stripping voltammetry;
D O I:
10.1016/j.talanta.2005.04.067
中图分类号:
O65 [分析化学];
学科分类号:
070302 ;
081704 ;
摘要:
An anodic stripping voltammetric method for the determination of cardiac troponin I (cTnI) at a MCM-41 mesoporous material modified carbon paste electrode (MCM-MCPE) was investigated. The test was based on the dual monoclonal antibody "sandwich" principle using colloidal gold as a labeled substrate. Four main steps were carried out to obtain the analytical signal, i.e. electrode preparation, immunoreaction, silver enhancement, and anodic stripping voltammetric detection. The anodic stripping peak current increased linearly with the concentration of cTnI over the range of 0.8-5.0 ng/ml. A detection limit of 0.5 ng/ml was obtained. The established method was applied to detect cTnI in acute myocardial infarction (AMI) samples using routine enzyme-linked immunoadsorbent assay (ELISA) for comparison analysis, and good results were obtained. (c) 2005 Elsevier B.V. All rights reserved.
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页码:61 / 66
页数:6
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