Single-molecule analysis of human telomerase monomer

被引：47

作者：

Alves, David ^{[1
]}

Li, Haitao ^{[1
]}

Codrington, Rosalind ^{[1
]}

Orte, Angel ^{[1
]}

Ren, Xiaojun ^{[1
]}

Klenerman, David ^{[1
]}

Balasubramanian, Shankar ^{[1
]}

机构：

[1] Univ Cambridge, Univ Chem Lab, Cambridge CB2 1EW, England

来源：

NATURE CHEMICAL BIOLOGY | 2008年 / 4卷 / 05期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1038/nchembio.82

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human telomerase is a ribonucleoprotein that is minimally comprised of protein (hTERT) and RNA (hTR) components. We have applied single-molecule fluorescence two-color coincidence detection to characterize complex formation between fluorophore-labeled components in solution. By systematic labeling and in vitro assembly of hTERT, hTR and telomerase's DNA substrate, we have established that catalytically functional human telomerase comprises a stable hTERT: hTR: substrate interaction in a 1:1:1 absolute stoichiometry.

引用

页码：287 / 289

页数：3

共 15 条

[1] N-terminal domains of the human telomerase catalytic subunit required for enzyme activity in vivo [J].

Armbruster, BN ;

Banik, SSR ;

Guo, CH ;

Smith, AC ;

Counter, CM .

MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (22) :7775-7786

[2] Functional multimerization of the human telomerase reverse transcriptase [J].

Beattie, TL ;

Zhou, W ;

Robinson, MO ;

Harrington, L .

MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (18) :6151-6160

[3] Tetrahymena telomerase is active as a monomer [J].

Bryan, TM ;

Goodrich, KJ ;

Cech, TR .

MOLECULAR BIOLOGY OF THE CELL, 2003, 14 (12) :4794-4804

[4] Protein composition of catalytically active human telomerase from immortal cells [J].

Cohen, Scott B. ;

Graham, Mark E. ;

Lovrecz, George O. ;

Bache, Nicolai ;

Robinson, Phillip J. ;

Reddel, Roger R. .

SCIENCE, 2007, 315 (5820) :1850-1853

[5] Dissociation among in vitro telomerase activity, telomere maintenance, and cellular immortalization [J].

Counter, CM ;

Hahn, WC ;

Wei, WY ;

Caddle, SD ;

Beijersbergen, RL ;

Lansdorp, PM ;

Sedivy, JM ;

Weinberg, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (25) :14723-14728

[6] IDENTIFICATION OF A SPECIFIC TELOMERE TERMINAL TRANSFERASE-ACTIVITY IN TETRAHYMENA EXTRACTS [J].

GREIDER, CW ;

BLACKBURN, EH .

CELL, 1985, 43 (02) :405-413

[7] Catalytically active human telomerase mutants with allele-specific biological properties [J].

Kim, M ;

Xu, LF ;

Blackburn, EH .

EXPERIMENTAL CELL RESEARCH, 2003, 288 (02) :277-287

[8] SPECIFIC ASSOCIATION OF HUMAN TELOMERASE ACTIVITY WITH IMMORTAL CELLS AND CANCER [J].

KIM, NW ;

PIATYSZEK, MA ;

PROWSE, KR ;

HARLEY, CB ;

WEST, MD ;

HO, PLC ;

COVIELLO, GM ;

WRIGHT, WE ;

WEINRICH, SL ;

SHAY, JW .

SCIENCE, 1994, 266 (5193) :2011-2015

[9] Ultrasensitive coincidence fluorescence detection of single DNA molecules [J].

Li, HT ;

Ying, LM ;

Green, JJ ;

Balasubramanian, S ;

Klenerman, D .

ANALYTICAL CHEMISTRY, 2003, 75 (07) :1664-1670

[10] Functional multimerization of human telomerase requires an RNA interaction domain in the N terminus of the catalytic subunit [J].

Moriarty, TJ ;

Huard, S ;

Dupuis, S ;

Autexier, C .

MOLECULAR AND CELLULAR BIOLOGY, 2002, 22 (04) :1253-1265

← 1 2 →