sgRNA Scorer 2.0: A Species-Independent Model To Predict CRISPR/Cas9 Activity

被引：169

作者：

Chari, Raj ^{[1
]}

Yeo, Nan Cher ^{[1
,2
]}

Chavez, Alejandro ^{[1
,2
]}

Church, George M. ^{[1
,2
]}

机构：

[1] Harvard Med Sch, Dept Genet, Boston, MA 02115 USA

[2] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

来源：

ACS SYNTHETIC BIOLOGY | 2017年 / 6卷 / 05期

关键词：

CRISPR; Cas9; sgRNA activity prediction; sgRNA scorer; genome engineering; STAPHYLOCOCCUS-AUREUS CAS9; OFF-TARGET; IN-VIVO; GENOME; CRISPR-CAS9; GENES; IDENTIFICATION; DESIGN; TOOL;

D O I：

10.1021/acssynbio.6b00343

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

It has been possible to create tools to predict single guide RNA (sgRNA) activity in the CRISPR/Cas9 system derived from Streptococcus pyogenes due to the large amount of data that has been generated in sgRNA library screens. However, with the discovery of additional CRISPR systems from different bacteria, which show potent activity in eukaryotic cells, the approach of generating large data sets for each of these systems to predict their activity is not tractable. Here, we present a new guide RNA tool that can predict sgRNA activity across multiple CRISPR systems. In addition to predicting activity for Cas9 from S. pyogenes and Streptococcus thermophilus CRISPR1, we experimentally demonstrate that our algorithm can predict activity for Cas9 from Staphylococcus aureus and S. thermophilus CRISPR3. We also have made available a new version of our software, sgRNA Scorer 2.0, which will allow users to identify sgRNA sites for any PAM sequence of interest.

引用

页码：902 / 904

页数：3

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