Gradient micropattern immobilization of EGF to investigate the effect of artificial juxtacrine stimulation

被引：75

作者：

Chen, GP

Ito, YY ^{[1
]}

机构：

[1] Univ Tokushima, Fac Engn, Dept Biol Sci & Technol, Tokushima 7708506, Japan

[2] Natl Inst Adv Interdisciplinary Res, 3D Tissue Engn Grp, Tsukuba, Ibaraki 3058562, Japan

[3] JST, PRESTO, Kyoto 6190237, Japan

来源：

BIOMATERIALS | 2001年 / 22卷 / 18期

关键词：

epidermal growth factor; pattern immobilization; gradient pattern; immobilized growth factor; juxtacrine stimulation;

D O I：

10.1016/S0142-9612(00)00432-4

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 [生物医学工程];

摘要：

To investigate the concentration effects of immobilized biosignal molecules by microscopic observation, a gradient micropattern immobilization technique using a photomask was devised. Epidermal growth factor (EGF) was chosen as the biosignal molecule and was conjugated with photoreactive polyallylamine. The EGF derivative was immobilized onto a polystyrene plate by UV irradiation in the presence of a gradient-micropattern photomask. Gradient micropattern immobilization of the EGF derivative on the polystyrene plate was confirmed by immunostaining with anti-EGF antibody. Chinese hamster ovary cells overexpressing EGF receptors were cultured on the micropatterned plate. Growth enhancement was observed only in dense EGF derivative immobilized regions. The present technique is useful for the investigation of concentration-dependent effects of immobilized biosignal molecules. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：2453 / 2457

页数：5

共 16 条

[1]

Solution-based assembly of metal surfaces by combinatorial methods [J].