Overexpression of protective antigen as a novel approach to enhance vaccine efficacy of Brucella abortus strain RB51

被引:73
作者
Vemulapalli, R [1 ]
He, YQ [1 ]
Cravero, S [1 ]
Sriranganathan, N [1 ]
Boyle, SM [1 ]
Schurig, GG [1 ]
机构
[1] Virginia Polytech Inst & State Univ, Ctr Mol Med & Infect Dis, Virginia Maryland Reg Coll Vet Med, Dept Biomed Sci & Pathobiol, Blacksburg, VA 24061 USA
关键词
D O I
10.1128/IAI.68.6.3286-3289.2000
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Brucella abortus strain RB51 is an attenuated rough strain that is currently being used as the official live vaccine for bovine brucellosis in the United States and several other countries. We reasoned that overexpression of a protective antigen(s) of B. abortus in strain RB51 should enhance its vaccine efficacy. To test this hypothesis, we overexpressed Cu/Zn superoxide dismutase (SOD) protein of B. abortus in strain RB51. This was accomplished by transforming strain RB51 with a broad-host-range plasmid, pBBR1MCS, containing the sodC gene along with its promoter. Strain RB51 overexpressing SOD (RB51SOD) was tested in BALB/c mice for its ability to protect against challenge infection with virulent strain 2308. Mice vaccinated with RB51SOD, but not RB51, developed antibodies and cell-mediated immune responses to Cu/Zn SOD, Strain RB51SOD vaccinated mice dcl:eloped significantly (P < 0.05) more resistance to challenge than those vaccinated with strain RB51 alone. The presence of the plasmid alone in strain RB51 did not alter its vaccine efficacy. Also, overexpression of SOD did not alter the attenuation characteristic of strain RB51.
引用
收藏
页码:3286 / 3289
页数:4
相关论文
共 32 条
[31]   The CcrM DNA methyltransferase is widespread in the alpha subdivision of proteobacteria, and its essential functions are conserved in Rhizobium meliloti and Caulobacter crescentus [J].
Wright, R ;
Stephens, C ;
Shapiro, L .
JOURNAL OF BACTERIOLOGY, 1997, 179 (18) :5869-5877
[32]   ENDOGENOUS GAMMA-INTERFERON MEDIATES RESISTANCE TO BRUCELLA-ABORTUS INFECTION [J].
ZHAN, YF ;
CHEERS, C .
INFECTION AND IMMUNITY, 1993, 61 (11) :4899-4901