Three-dimensional structure of a purple lipoxygenase

被引：139

作者：

Skrzypczak-Jankun, E ^{[1
]}

Bross, RA ^{[1
]}

Carroll, RT ^{[1
]}

Dunham, WR ^{[1
]}

Funk, MO ^{[1
]}

机构：

[1] Univ Toledo, Dept Chem, Toledo, OH 43606 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2001年 / 123卷 / 44期

关键词：

D O I：

10.1021/ja011759t

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Polyunsaturated fatty acid metabolism is governed primarily by two enzymes, prostaglandin H synthase and lipoxygenase. The crystal structure of the metastable product-oxidized purple form of soybean lipoxygenase-3 was determined at 2.0 Angstrom resolution. The data reveal that the chromophore corresponds to an iron-peroxide complex, a potential intermediate in the catalyzed reaction. A significant alteration of the iron site accompanies the formation of the complex. The structure, the first for a fatty acid-lipoxygenase complex, also reveals an unexpected mode of binding, and identifies amino acid residues that may play significant roles in catalysis, regio- and stereoselectivity.

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页码：10814 / 10820

页数：7

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