Involvement of Na+-leak Channel in Substance P-induced Depolarization of Pacemaking Activity in Interstitial Cells of Cajal

被引：34

作者：

Kim, Byung Joo ^{[4
]}

Chang, In Youb ^{[3
]}

Choi, Seok ^{[2
]}

Jun, Jae Yeoul ^{[2
]}

Jeon, Ju-Hong ^{[1
]}

Xu, Wen-Xie ^{[5
]}

Kwon, Young Kyu ^{[4
]}

Ren, Dejian ^{[6
]}

So, Insuk ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Med, Dept Physiol, Seoul, South Korea

[2] Chosun Univ, Coll Med, Dept Physiol, Kwangju, South Korea

[3] Chosun Univ, Coll Med, Dept Anat, Kwangju, South Korea

[4] Pusan Natl Univ, Sch Korean Med, Div Longev & Biofunct Med, Yangsan, South Korea

[5] Shanghai Jiao Tong Univ, Dept Physiol, Sch Med, Shanghai 200030, Peoples R China

[6] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2012年 / 29卷 / 3-4期

关键词：

Interstitial Cells of Cajal; Na+-leak channel; NALCN; Substance P; Gastrointestinal muscle; MURINE SMALL-INTESTINE; CATION CHANNEL; SMOOTH-MUSCLE; ELECTRICAL RHYTHMICITY; GASTROINTESTINAL-TRACT; RECEPTOR EXPRESSION; RESPIRATORY RHYTHM; TRP CHANNELS; NK1; RECEPTOR; ION-CHANNEL;

D O I：

10.1159/000338504

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Interstitial cells of Cajal (ICCs) are the pacemaking cells in the gastrointestinal muscles that generate the rhythmic oscillations in membrane potential known as slow waves. ICCs also mediate or transduce inputs from the enteric nervous system. Substance P (SubP) is a member of the family of mammalian tachykinin peptides that are predominantly released by enteric neurons. This study assessed the relationship of Na+-leak channel (NALCN) in the SubP-induced depolarization in pacemaking activity in the gastrointestinal tract. The patch-clamp technique for whole-cell recording was used in cultured cluster and single ICCs. Electrophysiological and pharmacological properties of SubP in ICC pacemaking activity were similar to those of NALCN. Reverse-transcription polymerase chain reaction, Western blotting, and immunohistochemistry all showed abundant and localized expression of NALCN messenger RNA and protein in mouse small intestine. NALCN is involved in the SubP-induced depolarization of intestinal pacemaking activity. The protein is a potential target for pharmacological treatment of motor disorders of the gut. Copyright (C) 2012 S. Karger AG, Basel

引用

页码：501 / 510

页数：10

共 56 条

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