Electrochemical DNA biosensor for analysis of wastewater samples

被引：87

作者：

Lucarelli, F ^{[1
]}

Kicela, A ^{[1
]}

Palchetti, I ^{[1
]}

Marrazza, G ^{[1
]}

Mascini, M ^{[1
]}

机构：

[1] Univ Florence, Diparitmento Chim Polo Sci, I-50019 Florence, Italy

来源：

BIOELECTROCHEMISTRY | 2002年 / 58卷 / 01期

关键词：

DNA biosensor; screen-printed electrodes; wastewater samples; Toxalert((R)) 100;

D O I：

10.1016/S1567-5394(02)00133-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The application of a disposable electrochemical DNA biosensor to wastewater samples is reported. The DNA biosensor is assembled by immobilising double-stranded calf thymus DNA on the surface of a disposable, carbon screen-printed electrode (SPE). The oxidation signal of the guanine base, obtained by a square wave voltammetric scan, is used as analytical signal. The presence of compounds with affinity for DNA is measured by their effect on the guanine oxidation. The comparison of the results with a toxicity test based on bioluminescent bacteria has confirmed the applicability of the method to real samples. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：113 / 118

页数：6

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