New cloning vectors with temperature-sensitive replication

被引：35

作者：

Phillips, GJ ^{[1
]}

机构：

[1] Iowa State Univ Sci & Technol, Dept Microbiol, Ames, IA 50011 USA

来源：

PLASMID | 1999年 / 41卷 / 01期

关键词：

D O I：

10.1006/plas.1998.1380

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A series of cloning vectors with conditional, temperature-sensitive replication that are selectable with ampicillin, chloramphenicol, and kanamycin has been constructed. These vectors are derivatives of a pSC101 mutant that can replicate only at low temperatures. The cloning vectors carry a number of unique restriction sites and provide for screening of recombinant plasmids by ct complementation. These vectors have proven useful for a variety of applications where conditional replication of a recombinant plasmid is desired. (C) 1999 Academic Press.

引用

页码：78 / 81

页数：4

共 36 条

[1] A 37X103 MOLECULAR-WEIGHT PLASMID-ENCODED PROTEIN IS REQUIRED FOR REPLICATION AND COPY NUMBER CONTROL IN THE PLASMID PSC101 AND ITS TEMPERATURE-SENSITIVE DERIVATIVE PHS1 [J].

ARMSTRONG, KA ;

ACOSTA, R ;

LEDNER, E ;

MACHIDA, Y ;

PANCOTTO, M ;

MCCORMICK, M ;

OHTSUBO, H ;

OHTSUBO, E .

JOURNAL OF MOLECULAR BIOLOGY, 1984, 175 (03) :331-347

[2] CONSTRUCTION AND PROPERTIES OF A FAMILY OF PACYC184-DERIVED CLONING VECTORS COMPATIBLE WITH PBR322 AND ITS DERIVATIVES [J].

BARTOLOME, B ;

JUBETE, Y ;

MARTINEZ, E ;

DELACRUZ, F .

GENE, 1991, 102 (01) :75-78

[3] ALLELIC EXCHANGE IN ESCHERICHIA-COLI USING THE BACILLUS-SUBTILIS SACB GENE AND A TEMPERATURE-SENSITIVE PSC101 REPLICON [J].

BLOMFIELD, IC ;

VAUGHN, V ;

REST, RF ;

EISENSTEIN, BI .

MOLECULAR MICROBIOLOGY, 1991, 5 (06) :1447-1457

[4] GENE DISRUPTION IN ESCHERICHIA-COLI - TCR AND KM(R) CASSETTES WITH THE OPTION OF FLP-CATALYZED EXCISION OF THE ANTIBIOTIC-RESISTANCE DETERMINANT [J].

CHEREPANOV, PP ;

WACKERNAGEL, W .

GENE, 1995, 158 (01) :9-14

[5] REVISED INTERPRETATION OF ORIGIN OF PSC101 PLASMID [J].

COHEN, SN ;

CHANG, ACY .

JOURNAL OF BACTERIOLOGY, 1977, 132 (02) :734-737

[6] CONSTRUCTION OF BIOLOGICALLY FUNCTIONAL BACTERIAL PLASMIDS IN-VITRO [J].

COHEN, SN ;

CHANG, ACY ;

BOYER, HW ;

HELLING, RB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (11) :3240-3244

[7] FTSZ IS AN ESSENTIAL CELL-DIVISION GENE IN ESCHERICHIA-COLI [J].

DAI, K ;

LUTKENHAUS, J .

JOURNAL OF BACTERIOLOGY, 1991, 173 (11) :3500-3506

[8] NEW CLONING VECTORS FOR INTEGRATION INTO THE LAMBDA ATTACHMENT SITE ATTB OF THE ESCHERICHIA-COLI CHROMOSOME [J].

DIEDERICH, L ;

RASMUSSEN, LJ ;

MESSER, W .

PLASMID, 1992, 28 (01) :14-24

[9] A SIMPLE AND EFFICIENT SYSTEM FOR THE CONSTRUCTION OF PHOA GENE FUSIONS IN GRAM-NEGATIVE BACTERIA [J].

DUCHENE, AM ;

PATTE, J ;

GUTIERREZ, C ;

CHANDLER, M .

GENE, 1992, 114 (01) :103-107

[10] Gene integration in the Escherichia coli chromosome mediated by Tn21 integrase (Int21) [J].

Francia, MV ;

Lobo, JMG .

JOURNAL OF BACTERIOLOGY, 1996, 178 (03) :894-898

← 1 2 3 4 →