DNA from pollen: principles and potential

被引:34
作者
Bennett, K. D. [1 ]
Parducci, L. [1 ]
机构
[1] Uppsala Univ, Geoctr, SE-75236 Uppsala, Sweden
关键词
ancient DNA; pollen; plant population history; fossil; DNA sequence; Holocene;
D O I
10.1177/0959683606069383
中图分类号
P9 [自然地理学];
学科分类号
0705 ; 070501 ;
摘要
This paper describes our recent extraction of ancient DNA (aDNA) from Holocene pollen and discusses the potential of the technique for elucidating timescales of evolutionary change. We show that plastid DNA is recoverable and usable from pollen grains of Scots pine Pinus sylvestris from 10 ka and 100 years ago. Comparison of the ancient sequences with modern sequences, obtained from an extant population, establish a first genetic link between modern and fossil samples of Scots pine, providing a genetic continuity through time. One common haplotype is present in each of the three periods investigated, suggesting that it persisted near the lake throughout the postglacial. The retrieval of aDNA from pollen has major implications for palaeoecology by allowing (i) investigation of population-level dynamics in time and space, and (ii) tracing ancestry of populations and developing phylogenetic trees that include extinct as well as extant taxa. The method should work over the last glacial oscillation, thus giving access to ancestry of populations over a crucial period of time for the understanding of the relationship between speciation and climate change.
引用
收藏
页码:1031 / 1034
页数:4
相关论文
共 34 条
[11]   The genetic origins of the Andaman Islanders [J].
Endicott, P ;
Gilbert, MTP ;
Stringer, C ;
Lalueza-Fox, C ;
Willerslev, E ;
Hansen, AJ ;
Cooper, A .
AMERICAN JOURNAL OF HUMAN GENETICS, 2003, 72 (01) :178-184
[12]   Using chloroplast DNA to trace postglacial migration routes of oaks into Britain [J].
Ferris, C ;
Oliver, RP ;
Davy, AJ ;
Hewitt, GM .
MOLECULAR ECOLOGY, 1995, 4 (06) :731-738
[13]   Holocene dynamics of the southern boreal forest in Sweden [J].
Giesecke, T .
HOLOCENE, 2005, 15 (06) :858-872
[14]   CHLOROPLAST DNA-SEQUENCE FROM A MIOCENE MAGNOLIA SPECIES [J].
GOLENBERG, EM ;
GIANNASI, DE ;
CLEGG, MT ;
SMILEY, CJ ;
DURBIN, M ;
HENDERSON, D ;
ZURAWSKI, G .
NATURE, 1990, 344 (6267) :656-658
[15]   Ancient plant DNA: review and prospects [J].
Gugerli, F ;
Parducci, L ;
Petit, RJ .
NEW PHYTOLOGIST, 2005, 166 (02) :409-418
[16]   Identification of birds through DNA barcodes [J].
Hebert, PDN ;
Stoeckle, MY ;
Zemlak, TS ;
Francis, CM .
PLOS BIOLOGY, 2004, 2 (10) :1657-1663
[17]   Geologically ancient DNA: fact or artefact? [J].
Hebsgaard, MB ;
Phillips, MJ ;
Willerslev, E .
TRENDS IN MICROBIOLOGY, 2005, 13 (05) :212-220
[18]   Neandertal DNA sequences and the origin of modern humans [J].
Krings, M ;
Stone, A ;
Schmitz, RW ;
Krainitzki, H ;
Stoneking, M ;
Paabo, S .
CELL, 1997, 90 (01) :19-30
[19]   Is a large-scale DNA-based inventory of ancient life possible? [J].
Lambert, DM ;
Baker, A ;
Huynen, L ;
Haddrath, O ;
Hebert, PDN ;
Millar, CD .
JOURNAL OF HEREDITY, 2005, 96 (03) :279-284
[20]   Rates of evolution in ancient DNA from Adelie penguins [J].
Lambert, DM ;
Ritchie, PA ;
Millar, CD ;
Holland, B ;
Drummond, AJ ;
Baroni, C .
SCIENCE, 2002, 295 (5563) :2270-2273