High-performance liquid chromatographic assay of dichlorvos, isocarbophos and methyl parathion from plant leaves using chemiluminescence detection

被引：85

作者：

Huang, GM

Ouyang, J ^{[1
]}

Baeyens, WRG

Yang, YP

Tao, CJ

机构：

[1] Beijing Normal Univ, Dept Chem, Beijing 100088, Peoples R China

[2] State Univ Ghent, Fac Pharmaceut Sci, B-9000 Ghent, Belgium

[3] Minist Agr, Inst Control Agrochem, Beijing 100026, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2002年 / 474卷 / 1-2期

关键词：

high-performance liquid chromatography; chemiluminescence; dichlorvos; isocarbophos; methyl parathion;

D O I：

10.1016/S0003-2670(02)01014-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic (HPLC) method has been developed for the detection of organic phosphorous insecticides (dichlorvos(DDVP), isocarbophos and methyl parathion). In addition to a traditional method using UV detection, we developed an alternative method based on chemiluminescent reaction of organic phosphorous insecticides with luminol and hydrogen peroxide. The sample containing organic phosphorous insecticides was injected into a Hypersil ODS column (5 mum 4.6 x 100 mm) and eluted with methanol:water (60:40 (v/v)). The chemiluminescence (CL) response is linear to the concentration of DDVP, isocarbophos and methyl parathion, respectively. Most metal ions and organic compounds tested do not interfere with the determination. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：21 / 29

页数：9

共 12 条

[1] SIMULTANEOUS DETERMINATIONS OF HISTAMINE AND N-TAU-METHYLHISTAMINE BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-CHEMILUMINESCENCE COUPLED WITH IMMOBILIZED DIAMINE OXIDASE [J].