Thermodynamically controlled separation of polyvalent 2-nm gold nanoparticle-oligonucleotide conjugates

被引：30

作者：

Lee, Jae-Seung

Seferos, Dwight S.

Giljohann, David A.

Mirkin, Chad A.

机构：

[1] Northwestern Univ, Dept Chem, Evanston, IL 60208 USA

[2] Northwestern Univ, Int Inst Nanotechnol, Evanston, IL 60208 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 16期

关键词：

D O I：

10.1021/ja800797h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We describe the synthesis of small (2-nm diameter) gold nanoparticles densely functionalized with thiolated DNA (DNA-Au NPs) and a method to separate these particles from excess free DNA after synthesis. The separation method utilizes the thermodynamically enhanced binding properties of 2-nm DNA-Au NPs, compared to free excess DNA, to selectively hybridize these small particles to larger (15-nm diameter) DNA-Au NPs and form aggregates that can be isolated by simple centrifugation. These 2-nm DNA-Au NPs are obtained in a 46% overall yield, have a high surface coverage of DNA (64.8 +/- 6.4 pmol/cm(2)), and as a result, exhibit increased melting temperatures and cooperative melting properties.

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页码：5430 / +

页数：3

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