Optical detection of single molecules

被引：383

作者：

Nie, SM ^{[1
]}

Zare, RN ^{[1
]}

机构：

[1] STANFORD UNIV, DEPT CHEM, STANFORD, CA 94305 USA

来源：

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE | 1997年 / 26卷

关键词：

fluorescence; confocal microscopy; near-field optics; DNA; molecular motors;

D O I：

10.1146/annurev.biophys.26.1.567

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent advances in ultrasensitive instrumentation have allowed for the detection, identification, and dynamic studies of single molecules in the condensed phase. This measurement capability provides a new set of tools for scientists to address important current problems and to explore new frontiers in many scientific disciplines, such as chemistry, molecular biology, molecular medicine, and nanostructured materials. This review focuses on the methodologies and biological applications of single-molecule detection based on laser-induced fluorescence.

引用

页码：567 / 596

页数：30

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