Bidirectional transport by distinct populations of COPI-coated vesicles

被引：351

作者：

Orci, L ^{[1
]}

Stamnes, M ^{[1
]}

Ravazzola, M ^{[1
]}

Amherdt, M ^{[1
]}

Perrelet, A ^{[1
]}

Sollner, TH ^{[1
]}

Rothman, JE ^{[1
]}

机构：

[1] MEM SLOAN KETTERING CANC CTR,CELLULAR BIOCHEM & BIOPHYS PROGRAM,NEW YORK,NY 10021

来源：

CELL | 1997年 / 90卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0092-8674(00)80341-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electron microscope immunocytochemistry reveals that both anterograde-directed (proinsulin and VSV G protein) and retrograde-directed (the KDEL receptor) cargo are present in coPI-coated vesicles budding from every level of the Golgi stack in whole cells; however, they comprise two distinct populations that together can account for at least 80% of the vesicles budding from Golgi cisternae. Segregation of antero-grade- from retrograde-directed cargo into distinct sets of COPI-coated vesicles is faithfully reproduced in the cell-free Golgi transport system, in which VSV G protein and KDEL receptor are packaged into separable vesicles, even when budding is driven by highly purified coatomer and a recombinant ARF protein.

引用

页码：335 / 349

页数：15

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