An isocratic high-performance liquid chromatographic method for determining the available lysine in foods

被引：6

作者：

Castillo, G ^{[1
]}

Sanz, MA ^{[1
]}

Serrano, MA ^{[1
]}

Hernandez, T ^{[1
]}

Hernandez, A ^{[1
]}

机构：

[1] UNIV ALCALA DE HENARES, DEPT NUTR & BROMATOL, ALCALA DE HENARES 28871, MADRID, SPAIN

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 1997年 / 35卷 / 09期

关键词：

D O I：

10.1093/chromsci/35.9.423

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the quantitative determination of the available lysine in foods based on the reaction of the ∈-amino group of lysine with fluorodinitrobenzene is developed. Derivatization, hydrolysis, and the chromatographic conditions for separation are studied. To establish the reliability of the proposed method, the accuracy and precision of the analysis are evaluated using pure proteins and food samples. The results show the proposed method to be both accurate and precise.

引用

页码：423 / 429

页数：7

共 33 条

[1] DETERMINATION OF AVAILABLE LYSINE IN FOODS [J].

BLOM, L ;

HENDRICKS, P ;

CARIS, J .

ANALYTICAL BIOCHEMISTRY, 1967, 21 (03) :382-+

[2] MEASUREMENT OF AVAILABLE LYSINE IN HEATED AND UNHEATED FOODSTUFFS BY CHEMICAL AND BIOLOGICAL METHODS [J].

BOCTOR, AM ;

HARPER, AE .

JOURNAL OF NUTRITION, 1968, 94 (03) :289-&

[3] PROBLEMS IN DETERMINATION OF FDNB-AVAILABLE LYSINE [J].

BOOTH, VH .

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1971, 22 (12) :658-&

[4]

Carpenter K. J., 1973, Nutrition Abstracts and Reviews, V43, P423

[5] ESTIMATION OF THE AVAILABLE LYSINE IN ANIMAL-PROTEIN FOODS [J].

CARPENTER, KJ .

BIOCHEMICAL JOURNAL, 1960, 77 :604-610

[6]

CHEFTEL JC, 1977, FOOD PROTEINS, P401

[7] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF EPSILON-PYRROLE-LYSINE IN PROCESSED FOOD [J].

CHIANG, GH .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1988, 36 (03) :506-509

[8] ESTIMATION OF LYSINE DAMAGE IN HEATED WHEY PROTEINS BY FUROSINE DETERMINATIONS IN CONJUNCTION WITH THE DIGESTION CELL TECHNIQUE [J].

DESROSIERS, T ;

SAVOIE, L ;

BERGERON, G ;

PARENT, G .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1989, 37 (05) :1385-1391

[9]

ELNOCKRASHY AS, 1965, STARKE, V17, P89

[10] RELATIONSHIP BETWEEN BLOCKED LYSINE AND CARBOHYDRATE-COMPOSITION OF INFANT FORMULAS [J].

EVANGELISTI, F ;

CALCAGNO, C ;

ZUNIN, P .

JOURNAL OF FOOD SCIENCE, 1994, 59 (02) :335-337

← 1 2 3 4 →