Molecular authentication of Alisma orientale by PCR-RFLP and ARMS

被引：37

作者：

Li, Xuexia ^{[1
]}

Ding, Xiaoyu ^{[1
]}

Chu, Bihai ^{[1
]}

Ding, Ge ^{[1
]}

Gu, Sun ^{[1
]}

Qian, Liang ^{[1
]}

Wang, Ying ^{[1
]}

Zhou, Qi ^{[1
]}

机构：

[1] Nanjing Normal Univ, Coll Life Sci, Jiangsu Key Lab Biodivers & Biotechnol, Nanjing, Peoples R China

来源：

PLANTA MEDICA | 2007年 / 73卷 / 01期

关键词：

Alisma orientate (Sam.) Juzep; Alismataceae; nrDNA ITS region; PCR-RFLP; ARMS; molecular authentication;

D O I：

10.1055/s-2006-951746

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

As a widely used medicinal plant, Alisma orientate is always a possible target for fraudulent labeling. The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (nrDNA) of the six species of genus Alisma were sequenced, and two variant sites were found to be specific for A. orientate. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis and amplification refractory mutation system (ARMS) analysis were applied to the ITS region for the identification of A. orientate. A restriction site for Pstl useful for PCR-RFLP analysis was detected and a pair of diagnostic primers DFZX-JB02S and DFZX-JB02X were designed for ARMS.

引用

收藏

页码：67 / 70

页数：4

相关论文

共 15 条

[1]

Aranishi Futoshi, 2005, Journal of Applied Genetics, V46, P69

[2]

HSP70-hom gene single nucleotide (+2763 G/A and+2437 C/T) polymorphisms in sarcoidosis [J].

Bogunia-Kubik, K ;

Koscinska, K ;

Suchnicki, K ;

Lange, A .

INTERNATIONAL JOURNAL OF IMMUNOGENETICS, 2006, 33 (02) :135-140

[3]

Effect of alisol B acetate, a plant triterpene, on apoptosis in vascular smooth muscle cells and lymphocytes [J].

Chen, HW ;

Hsu, MJ ;

Chien, CT ;

Huang, HC .

EUROPEAN JOURNAL OF PHARMACOLOGY, 2001, 419 (2-3) :127-138

[4]

Pharmacological evaluation of several major ingredients of Chinese herbal medicines in human hepatoma Hep3B cells [J].

Chou, CC ;

Pan, SL ;

Teng, CM ;

Guh, JH .

EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2003, 19 (05) :403-412

[5]

Authentication of stems of Dendrobium officinale by rDNA ITS region sequences [J].

Ding, XY ;

Xu, LS ;

Wang, ZT ;

Zhou, KY ;

Xu, H ;

Wang, YQ .

PLANTA MEDICA, 2002, 68 (02) :191-192

[6]

Molecular phylogenetics of Diseae (Orchidaceae): A contribution from nuclear ribosomal ITS sequences [J].

Douzery, EJP ;

Pridgeon, AM ;

Kores, P ;

Linder, HP ;

Kurzweil, H ;

Chase, MW .

AMERICAN JOURNAL OF BOTANY, 1999, 86 (06) :887-899

[7]

Determination of the effectiveness of components of the herbal medicine Toki-Shakuyaku-San and fractions of Angelica acutiloba in improving the scopolamine-induced impairment of rat's spatial cognition in eight-armed radial maze test [J].

Hatip-Al-Khatib, I ;

Egashira, N ;

Mishima, K ;

Iwasaki, K ;

Iwasaki, K ;

Kurauchi, K ;

Inui, K ;

Ikeda, T ;

Fujiwara, M .

JOURNAL OF PHARMACOLOGICAL SCIENCES, 2004, 96 (01) :33-41

[8]

Corni fructus as the major herb of Die-Huang-Wan for lowering plasma glucose in Wistar rats [J].

Liou, SS ;

Liu, IM ;

Hsu, SF ;

Cheng, JT .

JOURNAL OF PHARMACY AND PHARMACOLOGY, 2004, 56 (11) :1443-1447

[9]

Identification of botanicals and potential contaminants through RFLP and sequencing [J].

Lum, MR ;

Potter, E ;

Dang, T ;

Heber, D ;

Hardy, M ;

Hirsch, AM .

PLANTA MEDICA, 2005, 71 (09) :841-846

[10]

ANALYSIS OF ANY POINT MUTATION IN DNA - THE AMPLIFICATION REFRACTORY MUTATION SYSTEM (ARMS) [J].

NEWTON, CR ;

GRAHAM, A ;

HEPTINSTALL, LE ;

POWELL, SJ ;

SUMMERS, C ;

KALSHEKER, N ;

SMITH, JC ;

MARKHAM, AF .

NUCLEIC ACIDS RESEARCH, 1989, 17 (07) :2503-2516