Intracellular oxygen-sensitive phosphorescent probes based on cell-penetrating peptides

被引：55

作者：

Dmitriev, Ruslan I. ^{[1
]}

Zhdanov, Alexander V. ^{[1
]}

Ponomarev, Gelii V. ^{[2
]}

Yashunski, Dmitri V. ^{[2
]}

Papkovsky, Dmitri B. ^{[1
]}

机构：

[1] Natl Univ Ireland Univ Coll Cork, Dept Biochem, Cork, Ireland

[2] Russian Acad Sci, Inst Biomed Chem, Moscow 119992, Russia

来源：

ANALYTICAL BIOCHEMISTRY | 2010年 / 398卷 / 01期

基金：

爱尔兰科学基金会;

关键词：

Cellular oxygen; Intracellular oxygen-sensitive probe; Cell-penetrating peptides; Pt-porphyrins; Mitochondrial function; Hypoxia; INTERNALIZATION MECHANISMS; PHOTODYNAMIC THERAPY; RICH PEPTIDES; NITRIC-OXIDE; PROLINE-RICH; PC12; CELLS; DELIVERY; MITOCHONDRIA; TRANSDUCTION; GRADIENTS;

D O I：

10.1016/j.ab.2009.10.048

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Probing of molecular oxygen in mammalian cells is important for the analysis of mitochondrial function, metabolic responses, and energetic status of the cells. We describe a new panel of intracellular O-2-sensitive probes based on phosphorescent porphyrin dyes conjugated to cell-penetrating peptides. The probes comprising the uncharged derivatives of Pt(II)-coproporphyrin I covalently linked to positively charged TAT-derived peptides are shown to effectively load live mammalian cells without any transfection reagents. The probes work well with all cell types tested, show similar subcellular localization, and produce characteristic responses to cell stimulation with mitochondrial uncouplers and inhibitors. They provide a simple and versatile tool for O-2 monitoring in live cells and in tissue, and an alternative to the existing O-2 probes which require facilitated transport into the cell. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：24 / 33

页数：10

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