Instantaneous spatial light interference microscopy

被引：81

作者：

Ding, Huafeng ^{[1
]}

Popescu, Gabriel ^{[1
]}

机构：

[1] Univ Illinois, Beckman Inst Adv Sci & Technol, Dept Elect & Comp Engn, Quantitat Light Imaging Lab, Urbana, IL 61801 USA

来源：

OPTICS EXPRESS | 2010年 / 18卷 / 02期

基金：

美国国家科学基金会;

关键词：

DIGITAL HOLOGRAPHIC MICROSCOPY; PHASE MICROSCOPY; REFRACTIVE-INDEX; DYNAMICS; TRANSPARENT; TOMOGRAPHY; BLOOD; CELLS;

D O I：

10.1364/OE.18.001569

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We present Instantaneous Spatial Light Interference Microscopy (iSLIM) as a new quantitative phase method that combines the benefits of white light illumination in Zernike's phase contrast microscopy and phase stability associated diffraction phase microscopy. iSLIM is implemented as an add-on module to a commercial phase contrast microscope, and enables new features to quantitative phase imaging: diminished speckle effects due to white light illumination, multimodal investigation potential due to overlaying with other modalities of the microscope (e.g. fluorescence, DIC, phase contrast), and spectroscopic potential due to the broad band light. We show proof of principle results by multicolor phase imaging of microsphere and red blood cells, and dynamic imaging of nanoscale cell membrane fluctuations. (C) 2010 Optical Society of America

引用

页码：1569 / 1575

页数：7

共 25 条

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