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Genome analysis of dengue type-1 virus isolated between 1990 and 2001 in Brazil reveals a remarkable conservation of the structural proteins but amino acid differences in the non-structural proteins

被引：20
作者：
dos Santos, CND
Rocha, CFS
Cordeiro, M
Fragoso, SP
Rey, F
Deubel, V
Desprès, P
机构：
[1] Inst Biol Mol Parana, BR-81350010 Curitiba, Parana, Brazil
[2] Fundacao Oswaldo Cruz, Rio De Janeiro, Brazil
[3] LACEN PE, Lab Dr Milton Bezerra Sobral, Recife, PE, Brazil
[4] CNRS, UPR 9053, Lab Genet Virus, F-91198 Gif Sur Yvette, France
[5] Inst Pasteur, Unite Flavivirus Host Mol Interact, F-75724 Paris, France
来源：
VIRUS RESEARCH | 2002年 / 90卷 / 1-2期
关键词：
dengue virus; genome sequencing; molecular evolution;
D O I：
10.1016/S0168-1702(02)00180-6
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We have investigated the genetic diversity of dengue type-1 (DEN-1) virus in Brazil. The full nucleotide sequences of three DEN-1 virus isolated from DEN fever (DF) and DEN hemorrhagic fever patients in northeastern Brazil in 1997 (BR/97) and one from a DF patient in the south of Brazil in 2001 (BR/01) were compared to that of the reference strain BR/90 obtained in the city of Rio de Janeiro in 1990. Sequence analysis showed that the structural proteins were remarkably conserved between all isolates. A total of 27 amino acid changes occurred throughout the non-structural proteins. Among them, nine amino acid substitutions were specific of BR/97 and BR/01 isolates, indicating that in situ evolution of these strains had occurred. Within the BR/97 and BR/01 samples, some amino acid substitutions have been previously identified in DEN-1 virus strains sequenced so far, suggesting that recombination events might have occurred. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：197 / 205
页数：9
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