Antioxidants induce different phenotypes by a distinct modulation of signal transduction

被引：36

作者：

Della Ragione, F ^{[1
]}

Cucciolla, V ^{[1
]}

Criniti, V ^{[1
]}

Indaco, S ^{[1
]}

Borriello, A ^{[1
]}

Zappia, V ^{[1
]}

机构：

[1] Univ Naples 2, Sch Med, Dept Biochem & Biophys, I-80138 Naples, Italy

来源：

FEBS LETTERS | 2002年 / 532卷 / 03期

关键词：

resveratrol; hydroxytyrosol; pyrrolidine dithiocarbamate; antioxidant; apoptosis; signal transduction;

D O I：

10.1016/S0014-5793(02)03683-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Antioxidants are known to exert a preventive activity against degenerative diseases. Here, we investigated the mechanism of action of three antioxidants: resveratrol, which causes differentiation of HL-60 cells, and hydroxytyrosol and pyrrolidine dithiocarbarnate which, in the same model system, activate apoptosis. The expression profile of hydroxytyrosol-treated cells showed the up-regulation of several genes, including c-jun and egr1. Pyrrolidine dithiocarbamate activates both genes, while resveratrol increases uniquely egr1. A selective modulation of signalling pathway explained this, finding. All antioxidants upregulate Erk1/2, while only hydroxytyrosol and pyrrolidine dithiocarbamate activate c-Jun N-terminal kinase (JNK). Since JNK induces apoptosis by Bcl-2 phosphorylation, we investigated this event. Bcl-2 phosphorylation was increased by hydroxytyrosol and pyrrolidine dithiocarbamate and not by resveratrol. Our results indicate that the different phenotypical effects of antioxidants correlate with modulation of selective transduction pathways. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：289 / 294

页数：6

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