Optimization of the performance of the polymerase chain reaction in silicon-based microstructures

被引：113

作者：

Taylor, TB ^{[1
]}

WinnDeen, ES ^{[1
]}

Picozza, E ^{[1
]}

Woudenberg, TM ^{[1
]}

Albin, M ^{[1
]}

机构：

[1] PE APPL BIOSYST,FOSTER CITY,CA 94404

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 15期

关键词：

D O I：

10.1093/nar/25.15.3164

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have demonstrated the ability to perform real-time homogeneous, sequence specific detection of PCR products in silicon microstructures. Optimal design/ processing result in equivalent performance (yield and specificity) for high surface-to-volume silicon structures as compared to larger volume reactions in polypropylene tubes. Amplifications in volumes as small as 0.5 mu l and thermal cycling times reduced as much as 5-fold from that of conventional systems have been demonstrated for the microstructures.

引用

收藏

页码：3164 / 3168

页数：5

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