Recombinant canarypox virus vaccine co-expressing genes encoding the VP2 and VP5 outer capsid proteins of bluetongue virus induces high level protection in sheep

被引：77

作者：

Boone, Josh D.

Balasuriya, Udeni B.

Karaca, Kemal

Audonnet, Jean-Christophe

Yao, Jiansheng

He, Ling

Nordgren, Robert

Monaco, Federica

Savini, Giovanni

Gardner, Ian A.

MacLachlan, N. James ^{[1
]}

机构：

[1] Univ Calif Davis, Sch Vet Med, Equine Viral Dis Lab, Dept Pathol Microbiol & Immunol, Davis, CA 95616 USA

[2] Merital Ltd, Athens, GA 30601 USA

[3] Merial SAS, Biol Discovery Res, F-69007 Lyon, France

[4] Sanofi Pasteur, Toronto, ON, Canada

[5] Ist Zooprofilatt Sperimentale Abruzzo & Molise G, I-64100 Teramo, Italy

[6] Univ Calif Davis, Sch Vet Med, Dept Med & Epidemiol, Davis, CA 95616 USA

来源：

VACCINE | 2007年 / 25卷 / 04期

关键词：

bluetongue; canarypox virus; vaccine; sheep;

D O I：

10.1016/j.vaccine.2006.08.025

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We describe the development and preliminary characterization of a recombinant canarypox virus vectored vaccine for protective immunization of ruminants against bluetongue virus (BTV) infection. Sheep (n = 6) immunized with recombinant canarypox virus vector (BTV-CP) co-expressing synthetic genes encoding the two outer capsid proteins (VP2 and VP5) of BTV serotype 17 (BTV-17) developed high titers (40-160) of virus-specific neutralizing antibodies and were resistant to challenge with a field strain of BTV-17. In contrast, sheep (11 = 5) immunized with a commercial recombinant canarypox virus vector expressing the E and preM genes of West Nile virus were seronegative to BTV and developed pyrexia, lymphopenia, and extended, high-titered viremias following challenge exposure to the field strain of BTV-17. These data confirm that the BTV-CP vaccine may be useful for the protective immunization of ruminants against bluetongue, and it may avoid the problems inherent to live-attenuated (LA) BTV vaccines. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：672 / 678

页数：7

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