Polysaccharide and lipid composition of the brown seaweed Laminaria gurjanovae

被引：74

作者：

Shevchenko, N. M. ^{[1
]}

Anastyuk, S. D. ^{[1
]}

Gerasimenko, N. I. ^{[1
]}

Dmitrenok, P. S. ^{[1
]}

Isakov, V. V. ^{[1
]}

Zvyagintseva, T. N. ^{[1
]}

机构：

[1] Russian Acad Sci, Pacific Inst Bioorgan Chem, Far E Div, Vladivostok 690022, Russia

来源：

RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2007年 / 33卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

alginate; brown seaweed; fatty acids; fucoidan; glyceroglycolipid; laminaran; Laminaria gurjanovae; phospholipid;

D O I：

10.1134/S1068162007010116

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polysaccharide and lipid composition of the Pacific brown seaweed Laminaria gurjanovae is determined. Alginic acid is shown to be the main polysaccharide of its biomass (about 28%); it consists of mannuronic and guluronic acid residues at a ratio of 3: 1. The yield of water-soluble polymannuronic acid is low and does not exceed 1.1% of dry biomass. High laminaran content (about 22%) is found, whereas the yield of fucoidan is no more than 3.6%. Laminaran consists of two fractions, soluble and insoluble in cold water, their ratio is 2.5: 1. Insoluble laminaran is a practically linear 1,3-beta-D-glucan, and the soluble fraction was shown to be 1,3;1,6-beta-D-glucan. The oligosaccharide products of desulfation or partial acidic hydrolysis of fucoidan were studied by MALDI TOF MS; they were found to be fuco- and galactooligosaccharides. The fucoidan is suggested to be a highly sulfated partially acetylated galactofucan (Fuc/Gal is similar to 1: 1). The main lipid components of the dried L. gurjanovae are neutral lipids and glyceroglycolipids, whereas phospholipids are found in minor amounts. The main fatty acid components of lipids are 14:0, 16:0, 16:1 omega-7, 18:1 omega-7 and 18:2 omega-6 acids.

引用

页码：88 / 98

页数：11

共 36 条

[1]

Algin Clare K, 1993, IND GUMS POLYSACCHAR, P105, DOI DOI 10.1016/B978-0-08-092654-4.50010-3

[2] DISTRIBUTION OF PHOSPHATIDYLCHOLINE IN BROWN-ALGAE [J].

ARAKI, S ;

SAKURAI, T ;

OOHUSA, T ;

KAYAMA, M .

NIPPON SUISAN GAKKAISHI, 1989, 55 (11) :2049-2049

[3] Sulfated fucans, fresh perspectives: structures, functions, and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharide [J].

Berteau, O ;

Mulloy, B .

GLYCOBIOLOGY, 2003, 13 (06) :29R-40R

[4] ADAPTATION OF A MACRO-SCALE METHOD TO MICROSCALE FOR FATTY-ACID METHYL TRANS-ESTERIFICATION OF BIOLOGICAL LIPID EXTRACTS [J].

CARREAU, JP ;

DUBACQ, JP .

JOURNAL OF CHROMATOGRAPHY, 1978, 151 (03) :384-390

[5] The use of osazones as matrices for the matrix-assisted laser desorption/ionization mass spectrometry of carbohydrates [J].

Chen, P ;

Baker, AG ;

Novotny, MV .

ANALYTICAL BIOCHEMISTRY, 1997, 244 (01) :144-151

[6] A disaccharide repeat unit is the major structure in fucoidans from two species of brown algae [J].

Chevolot, L ;

Mulloy, B ;

Ratiskol, J ;

Foucault, A ;

Colliec-Jouault, S .

CARBOHYDRATE RESEARCH, 2001, 330 (04) :529-535

[7] A study of fucoidan from the brown seaweed Chorda filum [J].

Chizhov, AO ;

Dell, A ;

Morris, HR ;

Haslam, SM ;

McDowell, RA ;

Shashkov, AS ;

Nifant'ev, NE ;

Khatuntseva, EA ;

Usov, AI .

CARBOHYDRATE RESEARCH, 1999, 320 (1-2) :108-119

[8]

CHRISTIE WW, 1988, J CHROMATOGR, V447, P305

[9] GLYCOLIPIDS, PHOSPHOLIPIDS AND FATTY-ACIDS OF BROWN-ALGAE SPECIES [J].

DEMBITSKY, VM ;

ROZENTSVET, OA ;

PECHENKINA, EE .

PHYTOCHEMISTRY, 1990, 29 (11) :3417-3421

[10] COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].

DUBOIS, M ;

GILLES, KA ;

HAMILTON, JK ;

REBERS, PA ;

SMITH, F .

ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356

← 1 2 3 4 →