DO11.10 and OT-II T cells recognize a C-terminal ovalbumin 323-339 epitope

被引:264
作者
Robertson, JM
Jensen, PE
Evavold, BD
机构
[1] Emory Univ, Sch Med, Dept Microbiol & Immunol, Atlanta, GA 30322 USA
[2] Emory Univ, Sch Med, Dept Pathol & Lab Med, Atlanta, GA 30322 USA
关键词
D O I
10.4049/jimmunol.164.9.4706
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The OVB323-339 epitope recognized by DO11.10 (H-2(d)) and OT-II (H-2(b)) T cells was investigated using amino- and carboxy-terminal truncations to locate the approximate ends of the epitopes and single amino acid substitutions of OVA(323-339) to identify critical TCR contact residues of the OVA(323-339) peptide. DO11.10 and OT-II T cells are both specific for a C-terminal epitope whose tore encompasses amino acids 329-337. Amino acid 333 was identified as the primary TCR contact residue for both cells, and amino acid 331 was found to be an important secondary TCR contact residue; however, the importance of other secondary TCR contact residues and peptide banking residues differ between the cells. Additional OVA(323-339)-specific clones were generated that recognized epitopes found in the N-terminal end or in the center of the peptide. These findings indicate that OVA(323-339) can be presented by I-A(d) in at least three binding registers. This study highlights some of the complexities of peptide Ags such as OVA(323-330), which contain a nested set of overlapping T cell epitopes and MHC binding registers.
引用
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页码:4706 / 4712
页数:7
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