Visually evoked activity in cortical cells imaged in freely moving animals

被引：166

作者：

Sawinski, Juergen ^{[1
]}

Wallace, Damian J. ^{[1
]}

Greenberg, David S. ^{[1
]}

Grossmann, Silvie ^{[2
]}

Denk, Winfried ^{[2
]}

Kerr, Jason N. D. ^{[1
]}

机构：

[1] Max Planck Inst Biol Cybernet, Network Imaging Grp, D-72076 Tubingen, Germany

[2] Max Planck Inst Med Res, Dept Biomed Opt, D-69120 Heidelberg, Germany

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2009年 / 106卷 / 46期

关键词：

calcium imaging; head-mounted microscope; neuronal activity; two-photon; visual cortex; CELLULAR RESOLUTION; 2-PHOTON MICROSCOPE; RESPONSES; CORTEX; AWAKE; MICROARCHITECTURE;

D O I：

10.1073/pnas.0903680106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We describe a miniaturized head-mounted multiphoton microscope and its use for recording Ca2+ transients from the somata of layer 2/3 neurons in the visual cortex of awake, freely moving rats. Images contained up to 20 neurons and were stable enough to record continuously for > 5 min per trial and 20 trials per imaging session, even as the animal was running at velocities of up to 0.6 m/s. Neuronal Ca2+ transients were readily detected, and responses to various static visual stimuli were observed during free movement on a running track. Neuronal activity was sparse and increased when the animal swept its gaze across a visual stimulus. Neurons showing preferential activation by specific stimuli were observed in freely moving animals. These results demonstrate that the multiphoton fiberscope is suitable for functional imaging in awake and freely moving animals.

引用

页码：19557 / 19562

页数：6

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