High-speed, miniaturized fluorescence microscopy in freely moving mice

被引：276

作者：

Flusberg, Benjamin A. ^{[1
]}

Nimmerjahn, Axel ^{[1
]}

Cocker, Eric D. ^{[1
]}

Mukamel, Eran A. ^{[1
]}

Barretto, Robert P. J. ^{[1
]}

Ko, Tony H. ^{[1
]}

Burns, Laurie D. ^{[1
]}

Jung, Juergen C. ^{[1
]}

Schnitzer, Mark J. ^{[1
,2
]}

机构：

[1] Stanford Univ, James H Clark Ctr Biomed Engn & Sci, Stanford, CA 94305 USA

[2] Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 11期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1038/nmeth.1256

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A central goal in biomedicine is to explain organismic behavior in terms of causal cellular processes. However, concurrent observation of mammalian behavior and underlying cellular dynamics has been a longstanding challenge. We describe a miniaturized (1.1 g mass) epifluorescence microscope for cellular-level brain imaging in freely moving mice, and its application to imaging microcirculation and neuronal Ca2+ dynamics.

引用

页码：935 / 938

页数：4

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