Creating new fluorescent probes for cell biology

被引：1600

作者：

Zhang, J

Campbell, RE

Ting, AY

Tsien, RY

机构：

[1] MIT, Dept Pharmacol, Cambridge, MA 02139 USA

[2] MIT, Dept Chem, Cambridge, MA 02139 USA

[3] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

[4] Univ Calif San Diego, Howard Hughes Med Inst, La Jolla, CA 92093 USA

来源：

NATURE REVIEWS MOLECULAR CELL BIOLOGY | 2002年 / 3卷 / 12期

基金：

加拿大健康研究院; 美国国家卫生研究院;

关键词：

D O I：

10.1038/nrm976

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Fluorescent probes are one of the cornerstones of real-time imaging of live cells and a powerful tool for cell biologists. They provide high sensitivity and great versatility while minimally perturbing the cell under investigation. Genetically-encoded reporter constructs that are derived from fluorescent proteins are leading a revolution in the real-time visualization and tracking of various cellular events. Recent advances include the continued development of 'passive' markers for the measurement of biomolecule expression and localization in live cells, and 'active' indicators for monitoring more complex cellular processes such as small-molecule-messenger dynamics, enzyme activation and protein-protein interactions.

引用

收藏

页码：906 / 918

页数：13

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