OMSSA Parser: An open-source library to parse and extract data from OMSSA MS/MS search results

被引:26
作者
Barsnes, Harald [1 ]
Huber, Steffen [2 ,3 ]
Sickmann, Albert [2 ]
Eidhammer, Ingvar [1 ]
Martens, Lennart [4 ]
机构
[1] Univ Bergen, Dept Informat, N-5020 Bergen, Norway
[2] ISAS Inst Analyt Sci, Dortmund, Germany
[3] Tech Univ Dresden, Dresden, Germany
[4] EBI, EMBL Outstn, Cambridge, England
关键词
Bioinformatics; OMSSA; Protein identification; PEPTIDE IDENTIFICATIONS; MASS;
D O I
10.1002/pmic.200900037
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Protein identification by MS is an important technique in both gel-based and gel-free proteome studies. The Open Mass Spectrometry Search Algorithm (OMSSA) (http:// pubchem.ncbi.nlm.nih.gov/omssa) is an open-source search engine that can be used to identify MS/MS spectra acquired in these experiments. We here present a lightweight, open-source Java software library, OMSSA Parser (http://code.google.com/p/omssa-parser), which parses OMSSA omx result files into easy accessible and fully functional object models. In addition, we also provide examples illustrating the usage of our library.
引用
收藏
页码:3772 / 3774
页数:3
相关论文
共 14 条
[1]   Mass spectrometry-based proteomics [J].
Aebersold, R ;
Mann, M .
NATURE, 2003, 422 (6928) :198-207
[2]   AN APPROACH TO CORRELATE TANDEM MASS-SPECTRAL DATA OF PEPTIDES WITH AMINO-ACID-SEQUENCES IN A PROTEIN DATABASE [J].
ENG, JK ;
MCCORMACK, AL ;
YATES, JR .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1994, 5 (11) :976-989
[3]   A method for assessing the statistical significance of mass spectrometry-based protein identifications using general scoring schemes [J].
Fenyö, D ;
Beavis, RC .
ANALYTICAL CHEMISTRY, 2003, 75 (04) :768-774
[4]   Open mass spectrometry search algorithm [J].
Geer, LY ;
Markey, SP ;
Kowalak, JA ;
Wagner, L ;
Xu, M ;
Maynard, DM ;
Yang, XY ;
Shi, WY ;
Bryant, SH .
JOURNAL OF PROTEOME RESEARCH, 2004, 3 (05) :958-964
[5]   MascotDatfile: An open-source library to fully parse and analyse MASCOT MS/MS search results [J].
Helsens, Kenny ;
Martens, Lennart ;
Vandekerckhove, Joel ;
Gevaert, Kris .
PROTEOMICS, 2007, 7 (03) :364-366
[6]   Peptizer, a Tool for Assessing False Positive Peptide Identifications and Manually Validating Selected Results [J].
Helsens, Kenny ;
Timmerman, Evy ;
Vandekerckhove, Joel ;
Gevaert, Kris ;
Martens, Lennart .
MOLECULAR & CELLULAR PROTEOMICS, 2008, 7 (12) :2364-2372
[7]   Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search [J].
Keller, A ;
Nesvizhskii, AI ;
Kolker, E ;
Aebersold, R .
ANALYTICAL CHEMISTRY, 2002, 74 (20) :5383-5392
[8]   RScore: a peptide randomicity score for evaluating tandem mass spectra [J].
Li, FX ;
Sun, W ;
Gao, YH ;
Wang, J .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2004, 18 (14) :1655-1659
[9]   PRIDE: The proteomics identifications database [J].
Martens, L ;
Hermjakob, H ;
Jones, P ;
Adamski, M ;
Taylor, C ;
States, D ;
Gevaert, K ;
Vandekerckhove, J ;
Apweiler, R .
PROTEOMICS, 2005, 5 (13) :3537-3545
[10]  
MATTHIESEN R, 2007, METHOD MOL BIOL, P121